Publikationsserver der RWTH Aachen University
Überexpression von s-Adenosylhomocystein-Hydrolase (SAH) in Zellen der Epstein-Barr Virus-Genom positiven Zell-Linie B95-8
Abstract
dc:descriptionThe Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with tumors like Burkitt's lymphoma or Hodgkin's lymphoma. In this thesis, the overexpression of s-adenosylhomocystein hydrolase (SAH) was analyzed in EBV genome positive B95-8 cells. The focus of the examination has been on the signal transduction pathways which play a part in the activation of latent persistent EBV.The gene of SAH was brought in a fusion vector (pcDNA-AHCY) which was transfected in B95-8 cells by endocytosis. The successful transfection was proved by the evidence of ß lactamase on the vector pcDNA-AHCY.In pcDNA-AHCY transfected B95-8 cells an increased expression of the viral genes EA-D and BZLF1 was shown by immunofluorescence assay and PCR. The transfection with SAH led to an increase of phosphorylated and activated protein kinase C (PKC). The lytic cycle might be activated additionally using a PKC and PLA2 independent way.The rate of DNA synthesis and cell proliferation increased significantly in pcDNA-AHCY transfected B95-8 cells. A modulated expression of surface markers CD11a, CD19, CD23, CD30, CD40, CD44, CD54, and CD58 has not been detected. The clarification of reaction mechanisms to activate the lytic cycle is an important step for the development of substances to therapy EBV associated tumors.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2005
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Werth, Georg
- Contributors dc:contributor
-
- Ritter, Klaus
Subjects
dc:subject × 8Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger