High-throughput experimentation for microscale cultivations and recombinant protein expression

In this work, common high-throughput cultivation processes in microtiter plates are investigated with the BioLector technology. It is demonstrated that extensive monitoring of key parameters (i.e. biomass and product formation) in microscale cultivations enables a profound understanding and hence optimization of recombinant expression systems and high-throughput cultivation processes. New concepts to deal with issues of different growth kinetics of cultures in microtiter plates are elaborated and tested. Furthermore, a novel high-throughput experimentation platform for microscale cultivations is established by combining a pipetting robot with the BioLector, resulting in the Robo-Lector platform. Ultimately, the Robo-Lector can contribute to the envisioned paradigm shift in bioprocess development. This entails switching from rather empirical process development (low-throughput experimentation with no or unsophisticated monitoring) to high-throughput experimentation with very sophisticated yet simple monitoring to gain deeper knowledge of biological production systems.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2010

Author dc:creator

• Huber, Robert

Contributors dc:contributor

• Büchs, Jochen

• info:eu-repo/classification/ddc/570
• High throughput screening
• Fermentation
• Mikrotiter-Platte
• Rekombinantes Protein
• Biowissenschaften, Biologie
• Hochdurchsatz
• Hochdurchsatz-Screening
• rekombinante Proteine
• Proteinexpression
• Biolector
• high-throughput
• microtiter plate
• recombinant protein
• protein expression

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

eng

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114072%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51820