Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1121 for “"Protein Expression"”.
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Synthetic circular RNA for protein expression
… challenges in circularization, purification, and protein expression have impeded a thorough investigation of exogenous circRNA. By rationally designing ubiquitous accessory sequences to facilitate circularization, we engineered a permuted self-splicing intron that efficiently circularized RNAs up …
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Gene and Protein Expression in Human Gliomas
… therapeutic approaches. We have utilised gene expression microarrays and 2D differential in-gel electrophoresis (DIGE) with sequential tandem mass spectrometry (MS/MS) protein identification, to investigate the gene and protein expression profiles in a set of gliomas of varying grades, as well …
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Transient heterologous protein expression in green microalgae
Introduction: Recombinant proteins are produced using recombinant DNA technology and are widely used in numerous industrial applications, as well as innovative therapeutics. As the requirements of industries and pharma grow more complex chemically, traditional chemical engineering is, at times, …
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Altered protein expression patterns in oesophageal cancer
… was to identify membrane or membrane-associated proteins that are expressed at different levels in oesophageal tumour tissue when compared to normal tissue. The identification of such proteins would be an important step towards the development of better diagnostic and therapeutic strategies for …
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Protein expression and glycosylation in CHO cells
… cell culture process depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells …
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Overcoming long-standing challenges in recombinant protein expression
<p>The market for proteins has been constantly increasing for many different applications, such as protein-based drugs. However, protein production in E. coli, the most widely used recombinant host, has remained fundamentally the same since its inception. Here, we developed from the bottom up, a …
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Towards understanding EC coupling protein expression with age
… receptor (RyR1), perhaps due to a decrease in expression of the DHPR alpha1s subunit which has been demonstrated in rodents. Changes in DHPR beta1a subunit expression have also been reported in aging mice but not investigated in humans. The 12kDa FK506 binding protein (FKBP12) stabilizes RyR1 …
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Towards understanding EC coupling protein expression with age
… receptor (RyR1), perhaps due to a decrease in expression of the DHPR alpha1s subunit which has been demonstrated in rodents. Changes in DHPR beta1a subunit expression have also been reported in aging mice but not investigated in humans. The 12kDa FK506 binding protein (FKBP12) stabilizes RyR1 …
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RNA and protein expression patterns of the Drosophila XRCC2 Homolog
… genome is thought to have five recA like proteins: Rad51B, Rad51C, Rad51D, XRCC2 and XRCC3. In Drosophila Rad51/SpnA, XRCC3/SpnB, and Rad51 C/SpnD participate in homologous recombination repair. The function of DmRad51 D and DmXRCC2 are unknown. The goal of this project was to elucidate …
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Quantification of the Chemotaxis Protein Expression Levels in Bacillus Subtilis
The expression levels of CheD appear to be tuned to give the functional ratio of protein need to participate in adaptation. When the potential binding partners of CheD are deleted (cheC and all ten receptors), the protein levels of CheD drop significantly. This finding suggests that B. subtilis …
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Influence of intestinal inflammation on bacterial protein expression in monoassociated mice
… Aim of this PhD-project was to identify E. coli proteins involved in E. coli’s adaptation to the inflammatory conditions in the gut and to investigate whether these factors affect the host. Furthermore, the molecular basis for strain-specific differences between probiotic and harmful E. coli in …
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Differential Protein Expression and Butanol Production using <i>Clostridium beijerinckii</i>
… bacterial proteome in efforts to identifying key proteins that play role in the overall process of butanol fermentation. In the future we hope to genetically engineer microorganisms to efficiently carry out butanol fermentation at commercially significant levels. We were also able to find that the …
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The Role of Codon Usage in Regulating Protein Expression, Structure and Function
… has some biological significance. The rhythmic expression and the proper function of the Neurospora FREQUENCY (FRQ) protein are essential for circadian clock function. However, unlike most genes inNeurospora, frq exhibits non-optimal codon usage across its entire open reading frame (ORF). …
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Characterization and Development of Strategies for Altering Protein Expression in JSL1 Cells
… is a common mechanism for regulating gene expression in eukaryotic cells. This process of differentially including or excluding variable exons provides a means for increasing proteome complexity. Alternative gene splicing occurs in a cell specific manner and may be influenced by changes in …
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THERMOSTABLE EXOSHELLS AS TOOLS FOR RECOMBINANT PROTEIN EXPRESSION AND IN VIVO DELIVERY
We have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using a single technology. tES provides steric accommodation and charge complementation to GFPuv, horseradish peroxidase (HRP), and Renilla luciferase, and Photinus …
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Molecular diversity and coat protein expression of Sweet potato leaf curl virus
… of a fragment of the replication-associated protein gene (AC1). Ten of these begomoviruses were closely related to SPLCV and one was closely related to Sweet potato leaf curl Georgia virus (SPLCGV). These results suggest that in the US, SPLCV may be more common in sweetpotato genotypes than …
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Mitochondrial Protein Expression in Natural and Induced Forms of Canine Dilated Cardiomyopathy
In conclusion, mitochondrial proteins associated with essential metabolic pathways were found to be altered in the myocardium of dogs with DCM. The altered mitochondrial proteins were mainly related to oxidative phosphorylation, but also included other elements of primary energy and other types of …
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Repeated Zolpidem Treatment Effects on Sedative Tolerance, Withdrawal, mRNA Levels, and Protein Expression
… surface, and intracellular GABAAR subunits protein expression due to 7 days of i.p. injections of diazepam and zolpidem compared to vehicle. Based on previous research both groups should become tolerant to zolpidem’s sedative effects and show decreases of GABAAR subunits and increases in …
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ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY
… it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation …
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