Publikationsserver der RWTH Aachen University
Adhäsions- und Nidationsprophylaxe nach i.p.-Implantation von SCOV3.ip-Zellen in SCID-Mäuse mittels Icodextrin, Hyaluronsäure und physiologischer NaCl-Lösung im Vergleich
Abstract
dc:descriptionThe problem of postsurgical adhesion formation is still in focus of scientific investigations. Although no clinical standard has been established for the prevention or therapy till today, there are promising attempts to solve the problem. Currently, the most promising approach can be seen in the application of physical adhesive barriers. These solutions allow a separation between the injured peritoneal surfaces and neighbouring structures during the postoperative healing phase. Nevertheless, their clinical application is still limited to the prevention of adhesion formation. It is known from literature that the pathology of postoperative adhesion formation shows parallels to the formation of peritoneal metastases and tumour implantation. Therefore, it becomes obvious that the formation of peritoneal carcinosis as a result of ovarian carcinoma due to peritoneal adhesion of tumour cells is possible. The aim of the present animal study was to evaluate the efficacy of the three barrier products icodextrin (Adept®), hyaluronic acid (Hyalobarrier®-Gel) and physiologic saline on adhesion formation as well as on tumour recurrence after surgery of ovarian carcinoma. Forty SCID-mice (NOD.CB17-Prkdcscid/J) were enclosed into the study and randomly assigned to four experimental groups of ten animals each: group 1: Icodextrin (Adept®), group 2: hyaluronic acid (Hyalobarrier®-Gel), group 3: physiologic saline, group 4: control group (no treatment). Peritoneal trauma was induced by standardized peritoneal lesion of the right abdominal wall. Ovarian carcinoma cell line SCOV3.ip was injected intraabdominally in all animals to induce specific tumour load. Afterwards the animals were treated by intraabdominal injection of the barrier products. After 21 postoperative days all animals were relaparotomized for analyses. Adhesion formation and tumour growth were measured using well established scores. Moreover, the tumour weight was gaged and tumour samples were taken for histological studies. All test substances (icodextrin, hyaluronic acid and physiologic saline) reduced postoperative adhesion formation and tumour growth. In the control group adhesion formation and tumour load were significantly increased compared to all other experimental groups. In mice treated with hyaluronic acid adhesion formation was significantly reduced compared to the other groups. Focusing on the parameter tumour growth, hyaluronic acid showed the best results again, but no statistic differences could be found between icodextrin, hyaluronic acid and saline. Histologic evaluation of the tumour tissue samples showed positive results of peritoneal carcinosis in all cases. With the limitation of an animal study, the results allow to suppose that the liquid compounds icodextrin, hyaluronic acid and physiologic saline may be beneficial adjuvants for prevention of postoperative adhesion formation and tumour recurrence. Further studies will have to focus on the evaluation of other barrier methods as well as on the realisation of in vivo studies with patients.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2008
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Bekes, Inga
- Contributors dc:contributor
-
- Rimbach, Stefan
Subjects
dc:subject × 13Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger