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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"podocyte"”.
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… damage to glomerular epithelial cells called podocytes, a key cell type involved in glomerular filtration. Mutations in the ACTN4 gene, encoding an actin-crosslinking protein, are causative of late-onset familial FSGS in humans. We have developed a mouse model of FSGS by expressing a …
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Electron microscopic morphometry of podocyte foot process effacement as a tool to distinguish primary from secondary focal segmental glomerulosclerosis (FSGS)
… importance. In this study, we evaluated podocyte FPW and FPE in FSGS patients to investigate whether a significant difference exists between primary and secondary FSGS. Methods: Cases histologically diagnosed as FSGS in adult native renal biopsies over a 5-year period at Groote Schuur …
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An Account of Chemical and Mechanical Regulation of TRPC6 Channels in Podocytes
Podocytes play a dynamic role in regulating glomerular filtration. The focus here is on the regulatory mechanisms of podocyte expressed transient receptor potential 6 (TRPC6) channels, an ion channel implicated in certain forms of proteinuric kidney disease. TRPC6 channels are polymodal and can be …
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A Stem Cell-Based Strategy for Modeling Human Kidney Disease and Discovering Novel Therapeutics
… involve irreversible damage to kidney glomerular podocytes - the specialized epithelial cells that encase glomerular capillaries and regulate the removal of toxins and waste from blood. Therefore, the goal of this research proposal was to develop a novel strategy to protect or promote repair of …
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Indagini strutturali e quantitative durante lo sviluppo del rene mediante l’utilizzo di elaborazione immagini
… for long-term renal health. In recent years, the podocyte depletion hypothesis has emerged as an important concept in kidney pathology. Moreover, a variety of renal and urological abnormalities have been reported in subjects with chromosomal aberrations, such as Down syndrome and Beta Thalassemia. …
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A Glomerular Basement Membrane-on-a-Chip Model for Investigating Charge-Selective Transport Across the Human Filtration Barrier
… and hemocompatible dialysis membranes. AB 8/13 podocyte monocultures, ciGEnCs endothelial monocultures, and podocyte–endothelial co-culture chips were maintained under static conditions, with flow applied only during the charged dextran perfusion. Before perfusion, confocal imaging confirmed …
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A Non-Canonical Function for NLRP3 and AIM2 in Kidney Diseases
… Interestingly, AIM2 was detected primarily in podocytes in the glomerulus and distal tubules at a low level. In a mouse model of nephrotoxic serum (NTS)-mediated anti-GBM, Aim2-/- mice displayed increased glomerular cellular crescent (multilayered accumulation of activated parietal cells) …
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Molecular Responses to Desialylation in a Rat Model of Induced Glomerulopathy
… insight into specific molecular responses of podocytes to injury, in addition to demonstrating the versatility of this animal model for descriptive and mechanistic studies regarding podocyte sialobiology.
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Regulation of Fluid-phase Uptake in Podocytes by Albumin-associated Lipids
<p>I. Podocytes are specialized epithelial cells in the kidney glomerulus that play important structural and functional roles in maintaining the filtration barrier. In nephrotic syndrome, a major breakdown of the kidney filtration barrier associated with proteinuria, hyperlipidemia, and edema, …
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New predictive and diagnostic biomarkers for preeclampsia
… there was increased urinary concentrations of podocyte specific extracellular vesicles (EVs) in women diagnosed with PE. The excessive circulating concentrations of HbF in women with PE was correlated to the concentration of urinary podocyte specific EVs and proteinuria. It was concluded that …
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Nephrotic Syndrome and Glomerular Basement Membrane: Genetic Defect of the Laminin α5 Chain
… resulting in possible instability of the podocyte actin cytoskeleton.
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Glomerulosclerosis in the Col1a2-deficient mouse model : homotrimer pathogenesis and MMP expression
… within the renal mesangium, resulting in podocyte foot effacement and proteinuria. In this study we examine the pre- and post-translational expression of type I collagen and MMPs -2, -3, and -9 in wildtype, heterozygous and Col1a2-deficient glomeruli to determine whether the pathogenic …
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The Role of Von Hippel-Lindau Protein in the Glomerulus
… Cxcr4, whereas overexpression of Cxcr4 alone in podocytes of transgenic mice is sufficient to cause glomerular disease. Despite the development of glomerular disease in mice that overexpress Cxcr4, their disease was milder and lacked features of full-blown RPGN. The Vhlh gene encodes VHL protein …
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Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney
… into renal tubular epithelial cells, whereas podocyte maturation behaved inversely. In addition, the analysis revealed that the ECM protein laminin, in contrast to collagen IV, promotes the maturation of renal progenitor cells into renal tubular epithelial cells, although no difference was …
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Protective effects of regular exercise and Korea red ginseng against high-fat diet-increased renal injury in ᴅ-galactose-induced aging rats
… staining for synaptopodin and WT-1, well-known podocyte markers, revealed that HFD aggravates the loss of podocytes in renal glomeruli. However, the regular exercise restored all these renal changes in HFD plus GAL-treated rats. KRG inhibited AGEs and collagen cross-link at ten-fold less …
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Untersuchungen zur Pathogenese des Nephrotischen Syndroms: Herstellung einer konditional immortalisierten Podozyten-Zellinie
Die Podozyten sind in den letzten Jahren in das Zentrum der Erforschung der Funktion des glomerulären Filters der Niere gelangt. Mutationen in Proteinen der Schlitzmembran sind nicht nur für eine beträchtliche Zahl an angeborenen glomerulären Erkrankungen verantwortlich, sondern werden auch …
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Expression und Funktion von Brain-derived-neurotrophic-factor in humanen Podozyten
Der derzeitige Kenntnisstand betreffend des neuronalen Wachstumsfaktors BDNF beruht überwiegend auf neurobiologischen Untersuchungen. Daher bleibt die mögliche Bedeutung einer in jüngster Zeit beobachteten BDNF-Expression auch in nicht-neuronalen Geweben zukünftig weiter abzuklären. <br> <br>In …
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Charakterisierung von purinergen Rezeptoren an kultivierten Podozyten der Maus
Extrazelluläre Nukleotide wie ATP und UTP sind wichtige Regulatoren glomerulärer Funktionen. In der vorliegenden Arbeit wurde die Reaktion von kultivierten Mauspodozyten auf purinenerge Agonisten mit der Saug-Elektroden Technik und intrazellulärer Ca2+-Messung (Fura 2) beobachtet, die eine …
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Charakterisierung des Angiotensin II AT1-Rezeptors in humanen differenzierten Podozyten
Die vorliegende Arbeit hat auf verschiedenen Ebenen nicht nur erstmals die Expression von allen wesentlichen Bestandteilen des Renin-Angiotensin-Systems in humanen differenzierten Podozyten gezeigt, sondern auch die Funktionalität dieses RAS nachgewiesen. Es wurden hierzu besonders die …