Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"pili"”.
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Binding mechanism of K88ab pili produced by enterotoxigenic Escherichia coli
Binding of K88ab pili by brush border membrane and mucus from pig small intestine was characterized by inhibition assay and Western blot. In Western blot, K88ab pili were bound by two major brush border membrane polypeptides with molecular weight of 61,500 and 57,000 in addition to numerous minor …
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Type IV Pili-Dependent Secretion of Biofilm Matrix Material Proteins in Clostridium perfringens
… The goal of these experiments was to identify pilin mutants that are needed for secretion of the biofilm matrix and develop a functional model for a type II secretion system (T2SS) in C. perfringens. Protein tagging, western blot, and slot blot experiments were done to quantify protein …
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… mutants in three genes with homology to Type IV pilins, and we used the promoter system described above to complement the mutants. We analyzed each pilin for localization and expression, as well as tested each of the mutants for various phenotypes frequently associated with type IV pili (TFP) and …
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The production and characterization of monoclonal antibodies against K88 pili from porcine enterotoxigenic Escherichia coli
Call number: LD2668 .T4 1985 G733
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The production and characterization of monoclonal antibodies against K88 pili from porcine enterotoxigenic Escherichia coli
Call number: LD2668 .T4 1985 G733
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Régulation du c-di-GMP et rôle de ce messager secondaire dans la formation de pili de type IV chez Clostridium difficile.
… de doctorat ont permis de démontrer le rôle des pili de type IV (T4P) dans l’agrégation de C. difficile et la régulation de leur expression par un riborégulateur à c-di-GMP. Les riborégulateurs sont des structures ARN situées dans la région 5’UTR des gènes capables de réguler l’expression des …
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Chimera Ligand for Pili and Lectin A Protein Controls Antibiotic-Promoted Biofilm Formation, Swarming Motility, Tolerance and Persister Formation by Pseudomonas aeruginosa
… a class of chimera ligands that can bind to both pili and LecA protein of Pseudomonas aeruginosa to inhibit both swarming motility and biofilm formation. The potential adjuvant agents of these chimera ligands that can increase the effectiveness of antibiotics were demonstrated. In addition, the …
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Chemical Control and Understanding of Horizontal Gene Transfers, Drug-Resistance Development, and Filament and Biofilm Formation
… conjugation and transduction, involve type IV pili appendages on bacterial surfaces. Here, the three mechanisms of horizontal gene transfer are studied. Saturated farnesol derivatives show inhibition effect on tetracycline-enhanced plasmid transformation, ciprofloxacin-enhances PAPI-1 …
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Molecular Basis of Corynebacterium Diphtheriae Virulence and Infection In The Caenorhabditis Elegans Model Host
… primarily require proteinaceous fibers called pili, which mediate adherence to specific tissues. The type strain of C. diphtheriae possesses three distinct pilus structures, namely the SpaA, SpaD, and SpaH-type pili, which are encoded by three distinct pilus gene clusters. The pilus is …
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A New Class of Antibiotic That Prevents Drug Tolerance, Persistence, and Resistance By Controlling Emergence of Phenotypes.
… bacteria. We identified two proteins, type IV pili and lectin LecA as receptors for our molecules by different methods, including a new label-free assay based on bacterial motility sensing the environment, chemical inhibition of bacteriophage adsorption on pili appendages of bacteria, and …
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Spectroscopic Studies of Abiotic and Biological Nanomaterials: Silver Nanoparticles, Rhodamine 6G Adsorbed on Graphene, and c-Type Cytochromes and Type IV Pili in Geobacter sulfurreducens
… 6G adsorbed on graphene, and the type IV pili and c-type cytochromes produced by the dissimilatory metal-reducing bacterium Geobacter sulfurreducens. Although these systems are quite different in some ways, they can all be considered examples of nanomaterials. A nanomaterial is generally …
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Characterization of symbiotically important processes in Sinorhizobium meliloti
… important bacterial traits, type IVb pili and chemotaxis. Chapter 2 characterizes S. meliloti type IVb pili encoded by flp-1 and establishes their role in nodulation. Bundle-forming pili were visualized in wild-type cells, while cells lacking pilA1, the pilin-encoding gene, showed an …
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Conservation Of The Intracellular Bacterial Community Pathogenic Pathway In Urinary Tract Infection
… IBCs) within bladder facet cells. Type 1 pili, adhesive fimbriae, are necessary for UPEC binding and invasion of urothelial cells and formation of IBCs. UPEC ultimately disperse from the IBC, many with filamentous morphology, and proceed to infect other host urothelial cells. This work …
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Kuchunguza Matumizi ya Methali katika Jamii ya Wanyiramba
… za Kinyiramba huundwaje na zina muundo gani. Pili, utafiti huo ulichunguza matumizi ya methali na nafasi yake katika kujenga na kuimarisha maadili katika jamii. Kutokana na utafiti uliofanywa kwa kutumia njia ya hojaji na ushiriki nafsi imebainika kwamba ingawa methali ziko katika jamii ya …
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Maana za Majina ya Watu wa Pemba Katika Lugha ya Kiswahili: Uchunguzi Kifani
… maana za majina ya watu katika jamii ya Wapemba, pili kueleza mazingira yanayosababisha watu kupewa majina hayo katika jamii ya Wapemba na tatu kufafanua vigezo vinavyowaongoza watoa majina kwa wanaume na wanawake. Ili kufikia lengo kuu lililokusudiwa mbinu mbalimbali za utafiti zilihusika. Mbinu …
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… main causal agents of urinary tract infections. Pili are bacterial surface adhesive organelles that are critical virulence factors in the successful colonization of the urinary tract by uropathogenic E. coli. Pili are composite in structure and are formed from the polymerization of different …
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Natural and Synthetic Ligand-Binding Induce Different Pilin Assemblies in vitro and Control P. aeruginosa Bioactivities in vivo, and Development of Bacterial-Motility Enabled Binding Assays
… Fibrous appendages formed by assembly of pilin proteins on P. aeruginosa are surface proteins that are necessary for host colonization, adhesion on abiotic surfaces, controlling motilities, formation of biofilm, horizontal gene transfer and virulence production of the bacterium. Upon …
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Usawiri Wa Mwanamke Kama Kiongozi Katika Tamthiliya: Uchunguzi Wa Kivuli Kinaishi Na Nguzo Mama
… nilijibu kuwa; nawaandikia wote ili waelimike. Pili, Mama yangu mpendwa Dkt Rose Cleopa Mapunjo Mpembeni kwa ushauri wake wa mara kwa mara kuhusu umuhimu wa elimu. Nawapenda wote na Mungu awabariki.
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Matatizo Wanayoyapata Wanafunzi Walibya katika Kujifunza Viambishi vya Njeo katika Lugha ya Kiswahili
… yanayohusiana na ufundishaji wa lugha ya pili. Sura ya Pili inachunguza kwa undani maumbile muhimu ya sarufi ya Kiswahili, hususani maumbo yanayojitokeza kuhusiana na viambishi vya njeo na maana zinazojitokeza zinazofanya matumizi yenye viambishi hivi kuwatatiza wanafunzi. Sura ya Tatu …
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Molecular Insights into the Uropathogenesis of Enterococcus faecalis and Escherichia coli in the Context of Urinary Catheterization
… murine bladders, it was shown that type 1 pili mediate UPEC adherence and invasion, similar to what was observed in non-catheterized bladders. Studies in this murine model further demonstrated that microbial reservoirs established during previous UPEC infection could serve as a nidus for …
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