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Showing 1 to 14 of 14 for “"microscope objective"”.
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Design Of A Dynamic Focusing Microscope Objective For Oct Imaging
… with LC lenses, one involving a reflective microscope objective sub-system, and the other involving an all-refractive immersion microscope objective sub-system are investigated. In order to ensure high resolution imaging, a low coherence broadband source, namely a femtosecond mode-locked Ti: …
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Custom built atomic force microscope for nitrogen-vacancy diamond magnetometry
… and collection of fluorescence with a high-NA objective from the same side. The AFM has a large open bottom and top and thus provides dual optical access. The motion of the cantilever is measured by optical beam deflection so that a wide range of commercial cantilevers can be used. The AFM and …
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In-situ backplane inspection of fiber optic ferrules
… of a single daughter card, moves a custom microscope objective in three degrees of freedom to image the connector endfaces, and detects and classifies defects with major diameter of one micron or larger.
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Wavelength swept spectrally encoded confocal microscopy for biological and clinical applications
… mounted mirror and a 0.9 NA water immersion microscope objective.
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Design of a liquid embossing machine
… by optical feedback using a camera and microscope objective. The theory of liquid embossing is discussed. PDMS has a very high contact angle with most fluids and so it is able to clear liquid from channels approximately 5 [mu]m wide. The wettability of the substrate is modified after …
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IMAGING INTERFEROMETRIC MICROSCOPY TO THE LIMITS OF AVAILABLE FREQUENCY SPACE
… illumination setup, while using a low NA microscope objective and thus keeping the large working distance, depth of focus and field of view associated with the lower NA. The goal of this dissertation is to reach ultimate resolution limits of non-fluorescent microscopy by using IIM in new …
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Development of the in vivo flow cytometer
… focused across a blood vessel by an achromat and microscope objective. Fluorescent cells are excited one by one as they flow through the excitation laser light slit, creating a burst of fluorescence whose width is inversely proportional to their velocity. The fluorescence signal is detected …
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Resolution improvement in optical microscopy by use of multi-beam interferometric illumination
… microscopy, the numerical aperture (NA) of the objective lens intrinsically links the resolution, depth of field (DOF), working distance (WD) and field of view (FOV). In a diffraction-limited system, changing the NA to improve resolution is not possible without degrading the other optical …
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Computational control of laser systems for micro-machining
… was also achieved with this system using a microscope objective lens to produce smaller voxels ranging from 5 to 10 pm in soda-lime glass, fused silica and sapphire samples. The C02 laser (1.5 kW, 10.6 pm, minimum pulse width of 26 ps) was used to fabricate microchannels in soda-lime glass …
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Application of beam shaping in second-harmonic generation microscopy
… The first approach incorporates a reflective microscope objective and a Bessel-Gauss beam illumination to reduce chromatic dispersion in SHG imaging. Using well-aligned, densely populated samples of collagen fibers, the performance of the reflective objective is systemically compared with a …
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Development and Evaluation of Whole Slide Hyperspectral Confocal Fluorescence and Brightfield Macroscopy
… f-theta laser scan lens thus allowing an entire microscope slide (22x70 mm) to be imaged in a single scan at resolution equivalent to a 10x microscope objective. The development and optimization of brightfield and single-channel fluorescence imaging modes are discussed in the first half of this …
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Simultaneous Immersion Mirau Interferometry
… has been developed. A custom attachment for a microscope objective has been designed and built for interferometric imaging with the possibility of immersion of the apparatus into cell medium. The implemented data collection algorithm is based on the principles of Phase-Shifting Interferometry. …
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New Applications of Fluorescence Correlation Spectroscopy in Materials Science
… species was developed. The adaptation of the microscope optics to non-aqueous environments was done by replacing the present microscope objective by a multi-immersion objective. Secondly, a sample chamber was developed that was not only resistant to organic solvents in all parts but also …
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Liquid Crystal Optics For Communications, Signal Processing And 3-d Microscopic Imaging
… A no-moving-parts axial scanning confocal microscope (ASCM) system is designed and demonstrated using a combination of a large diameter LC lens and a classical microscope objective lens. By electrically controlling the 5 mm diameter LC lens, the 633 nm wavelength focal spot is moved …