Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 105 for “"T-DNA"”.
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… that provides for strictly regulated T-DNA transfer from agrobacteria to model plants Nicotiana benthamiana. The study provided detailed information about the relative activity and regulation of several IPTG and cumic acid inducible promoters in A. tumefaciens. Additionally, new …
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Characterization of T-DNA integration sites within a population of insertional mutants of the diploid strawberry Fragaria vesca L.
… Fragaria vesca has facilitated the use of a T-DNA mutagenesis system to develop a collection of several hundred insertional T-DNA mutants at Virginia Tech, using either of two commercially available vectors, pCAMBIA 1302 and 1304. In this study, we have used expression of the green fluorescent …
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Improvement of a high throughput method for identification of T-DNA insertion events in an activation-tagged population of Populus tremula x Populus alba.
… an improved method to characterise the random T-DNA insertions sites of the activation tagged Populus tremula x Populus alba population generated by the Regan lab. In this thesis, I explore the potential of using a capture-based sequencing method to construct a high-throughput sequencing method …
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Amino Acid Transporters in Arabidopsis Thaliana
… ART1, were identified from the Arabidopsis EST cDNA library by complementation. LHT1 is a lysine and histidine transporter, and ART1 is an arginine and aromatic amino acid transporter. ART1 seemed to possess transport activity for IAA and other aromatic amino acid derivatives. Two T-DNA tagged …
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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a …
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Identifizierung und Analyse der an der pflanzlichen Zellwandbiosynthese beteiligten Gene in Arabidopsis thaliana
… beteiligt ist, an einer Arabidopsis thaliana T-DNA Knockout Mutante untersucht. Es ließ sich nachweisen, dass die T-DNA Insertion im UGE3-Gen (UDP-Glukose Epimerase), einem Gen des Nukleotidzucker-Synthesewegs, erfolgt war. Die Mutante zeigt eine starke Verkürzung der Wurzellänge. Die …
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Genetic studies of phenotypic variants in the woodland strawberry, (Fragaria vesca)
… forward genetics studies evaluating putative T-DNA insertional mutants in F. vesca. The observed phenotypes include alterations to floral development, anthocyanin pigmentation and leaf structure. The floral development mutant named green petal (gp) was not associated with the T-DNA insertions …
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Characterisation of the AT4G11100 gene, a negative regulator of disease resistance in Arabidopsis thaliana
… of 8 known genes in the region. Analysis of T-DNA knockouts of these 8 genes suggests that AT4G11100 is the mostly likely candidate for CIR1. This project established that the disease resistance phenotype of cir1 is temperature dependent and linked to reduced plant growth. Genetic crosses …
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Struktur und Funktion des GR1-Gens aus Arabidopsis thaliana (L.) Heynh
… and NOCO. The gr1 mutant was isolated from a T-DNA population of Ws-0 plants showing susceptibility towards the WELA isolate, however, retaining resistance towards the NOCO isolate. Since it seemed that the T-DNA insertion into the GR1 gene was the cause for the loss of resistance against the …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
A novel strategy for site-specific DNA transformation of plants has been proposed and the first component of the system developed. The proposed method overcomes the limitations of current techniques by providing a specific integration site for the insertion of transgenes using features of the …
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Characterization of sugar-insensitive mutants and analysis of sugar-regulated gene expression in Arabidopsis thaliana
… and is not linked to the presence of a T-DNA insert carried by the mutant. The sis3 mutation maps to the bottom arm of chromosome 3. The sis3 mutant is slightly insensitive to the effects of exogenously applied abscisic acid (ABA) on seed germination and root elongation but has a wild-type …
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Molecular and Physiological Analysis of Putative Amino Acid Transporters in Arabidopsis Thaliana
… plant Arabidopsis thaliana. A valine resistant T-DNA insertion mutant was first analyzed for this purpose. The mutant plant was isolated after screening 4900 T-DNA insertion mutants by growing them on media containing toxic (1 mM) concentration of valine (Chen, 1997). The mutant was also resistant …
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Genetic and phenotypic characterisation of an Arabidopsis thaliana jasmonic acid signalling mutant, jam2
… acid modified2 (jam2), was isolated from T-DNA activation tagged lines and characterized genetically and phenotypically. jam2 was found not to be T-DNA tagged as the T-DNA segregates independently of the mutation. jam2 is unlikely to be an anthocyanin biosynthetic mutant but shows delayed …
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KINETIC ANALYSIS OF AUXIN-INDUCED TRANSCRIPTION FACTOR NETWORKS CONTROLLING LATERAL ROOT DEVELOPMENT IN ARABIDOPSIS THALIANA
… factors from ARF19. Quantitative RT-PCR and T-DNA mutant phenotype analysis support their regulation by ARF19 and roles in lateral root development. Using literature and in silico relationships between all transcription factors, 12 transcription factors were identified from this network to …
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Charakterisierung des GR1-Gens aus Arabidopsis thaliana (L.) Heynh. im Hinblick auf die rassenspezifische Resistenz des Ökotypen Wassilewskija gegenüber dem Peronospora parasitica-Isolat WELA
… the susceptible gr1 mutant GR1 is tagged by a T-DNA insert and it was important to establish whether the mutant phenotype was indeed due to the T-DNA insertion in GR1. Therefore, gr1 plants were retransformed using different genomic constructs. Independent transformant lines showed local …
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Characterization of the cardiolipin synthase from arabidopsis thaliana
… mitochondria, A. thaliana mutants carrying a T-DNA insertion in four different positions of the CLS gene were analysed. Plants homozygous with respect to the insertion in either the CLS promoter region or the intron 7 of the CLS gene showed no significant alterations in the CL levels. On the …
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Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
… ternary vector system aimed at enhancing T-DNA delivery. Medium with phytohormone profiles was tested to reduce the regeneration process. The new method drastically reduced the total transformation process to 51 days while maintaining a comparable transformation frequency. Our study …
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Phytol aus dem Chlorophyllabbau ist limitierend für die Tocopherol (Vitamin E)-Synthese
… katalysiert, wurden zwei unabhängige T-DNA-Insertionsmutanten für Chlorophyllase1 (CHL1) und eine T-DNA-Insertionsmutante für Chlorophyllase2 (CHL2) identifiziert und eine chl1-1chl2-Doppelmutante erzeugt. Die Analyse der Chlorophyllidanteile ergab eine im Vergleich zum Wildtyp starke …
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Exploring the origins of DNA using synthetic biology Alannah Marie
The extreme conservation of DNA makes it difficult to trace the deep evolution of this fundamental molecule. The precursors of DNA are produced from the constituent RNA precursors through ribonucleotide reduction, leading to the logical conclusion that DNA evolved after RNA. Thymine (T) is produced …
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