Charakterisierung des GR1-Gens aus Arabidopsis thaliana (L.) Heynh. im Hinblick auf die rassenspezifische Resistenz des Ökotypen Wassilewskija gegenüber dem Peronospora parasitica-Isolat WELA

In this study the role of the GR1 gene in the race-specific resistance of the Arabidopsis ecotype Ws to the P. parasitica isolate WELA was investigated. In the susceptible gr1 mutant GR1 is tagged by a T-DNA insert and it was important to establish whether the mutant phenotype was indeed due to the T-DNA insertion in GR1. Therefore, gr1 plants were retransformed using different genomic constructs. Independent transformant lines showed local resistance responses after inoculation with WELA and these were not apparent in control plants. However, a completely resistant transformant was not observed. An 8 kb genomic fragment containing the GR1 gene was sequenced. The predicted GR1 protein has a 57% similarity to the ILR1 protein (Bartel und Fink, 1995), but not the same enzyme activity. GR1 transcripts accumulated in leaves after inoculation with WELA, wounding or treatment with methyljasmonate. However, transcripts did not accumulate after exposure to extreme temperatures, infection with P. syringae pv. maculicola, or application of salicylic acid. It was shown that the GR1 protein interacts with 14-3-3 proteins in a yeast-two-hybrid-assay, suggesting a potential mechanism for GR1-mediated signal transmission. The analysis of the GR1 gene and its product will lead to the identification of further components of signal pathways important in disease resistance and improve our understanding of race-specific signal transduction in plant defence.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2001

Author dc:creator

• Kruse, Cordula Maria

Contributors dc:contributor

• Slusarenko, Alan

• info:eu-repo/classification/ddc/570
• Biowissenschaften, Biologie
• Ackerschmalwand
• Resistenzgen
• Peronospora parasitica
• Struktur-Aktivitäts-Beziehung

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118618%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56523