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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Restriction analysis"”.
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Restriction Analysis of Bacteriophage
<p>A restriction map of DNA from bacteriophage P3-C<sub>1</sub> for the endonucleases BamH I, Bgl II, EcoR I, Hind III, Sac I and Sca I is described in this study. The phage DNA was determined to be a linear double stranded molecule with a unit genomic length of 31 kb. After electrophoresis in 0.5% …
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A Restriction Analysis of Klebsiella Pneumoniae Phage SBS
… with a battery of endoncleases and partial restriction maps determined. The potential of the phage as a vector for Klebsiella is discussed in the text. </p>
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role of NF-<i>k</i>B signaling in multiple myeloma
… through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the …
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Human intestinal microbiota and metabolites they produce in relation to host health
… of the 38 strains using amplified ribosomal DNA restriction analysis (ARDRA) into subspecies indicated that of the 38 bifidobacterial strains tested, 34 were designated B. longum subsp. longum and four B. longum subsp. infantis.
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Investigation of a ribosomal RNA operon of Frankia sp. strain AcN14a.
… different set of rRNA genes, were identified by restriction analysis. The rRNA genes of Frankia AcN14a have been compared to those of E. Coli and other eubacterial species. Frankia AcN14a rRNA genes show over 98% idenity to those of Frankia ORS020606 and usually show over 80% identity to other …
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Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)
… in the gut of R. flavipes using amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyzed in the ARDRA study, 261 different ARDRA …
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Aberrant DNA methylation as a diagnostic and predictive marker of ovarian cancer
… PCR (MSP) and Combined Bisulphite Restriction Analysis (COBRA) methods. CGI methylation of at least one of these loci was a frequent event in both early (78%) and late stage (60%) disease. A group of loci were identified as being methylated in 64% of early stage tumours (CGIs linked …
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Characterization of Dna Complementary to Rabbit Cytochrome P-450 Messenger-Rnas and Studies on Their Differential Regulation by Phenobarbital (Gene, Expression)
… of plasmids were found in these 20 plasmids by restriction analysis. Sequencing of these plasmids revealed that these clones were 50-60% homologous to rat phenobarbital-type cytochrome P-450. Based on comparison to the rat sequence, two of the cDNAs are missing sequence coding for the first 10 …
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The Biological Burden of Antibiotic Resistance in Staphylococcus aureus
… for 28 days, through 56 transfers. Using restriction analysis and DNA sequencing, we found that the final population was highly enriched for mutations in a 3 kb region of the plasmid corresponding to the plactamase operon and two ORFs with homology to the abortive infection protein AbiK of …
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DNA systematic studies in the genus Bromus L. (Poaceae)
Chloroplast and ribosomal DNA restriction endonuclease analyses were used to assess phylogenetic relationships between different subgenera of Bromus, a genus of over 100 diploid and polyploid species. Variation in chloroplast DNA (cpDNA) fragment pattern was examined, initially, in 15 species of …
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Molecular biology and biochemistry of brain tumours
… chain reaction (RT-PCR), restriction digestion analysis and direct sequencing demonstrated expression of CCK peptide itself and its A and B receptors by human gliomas, meningiomas and pituitary tumours. CCK peptides stimulated growth of cultured gliomas and meningiomas as …
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Extracellular Electron Shuttle Mediated Biodegradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine
… were characterized using amplified 16S rDNA restriction analysis. The data demonstrated that a novel Fe(III)-reducing community develops as RDX is degraded. This study also led to isolation and characterization of a novel species from RDX contaminated aquifer material that is capable of …
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Iron reduction mediated increases in carbon oxidation and phosphorus precipitation in on-site wastewater systems
… diversity of the wastewater. Amplified 16S rDNA restriction analysis (ARDRA) confirmed that unique Fe3+-reducing microbial communities increased significantly in iron-amended incubations. Pure culture growth experiments in ferric citrate media with Geobacter metallereducens clearly showed …
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Identificación taxonómica y clonal de bacterias acéticas, y estudio del efecto de la nisina frente a biofilms de bacterias enológicas
… In this thesis methodologies based on DNA analysis have been developed for clonal and taxonomic identification of AAB isolates from samples of wine- cider- and ethanol vinegars in full acetic fermentation. Moreover, the methodology of fluorescence microscopy has been developed for …
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Meiofauna in a chemosynthetic groundwater ecosystem: Movile Cave, Romania
… anhand von Amplified Ribosomal DNA Restriction Analysis (ARDRA) zeigten, dass die bakterielle Diversität der schwimmenden mikrobiellen Matten in Movile möglicherweise höher als erwartet ausfällt. Zusammenfassend lässt sich festhalten, dass unsere Untersuchungen eine erste Skizze der …
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Monitoring lactococcal phages during milk fermentation
… of sequence data from the purR and epsD analysis allowed the identification of 12 genetic lineages in Blend X. The presence of lactococcal phages in the fermentation industry can have detrimental effect on both the acidification capacity and the quality of the final product. Blend X is …
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Caractérisation moléculaire des communautés microbiennes associées à la rhizosphère de picea glauca non génétiquement modifiés et génétiquement modifiés avec le transgène BT(CryIa(b))
… été sujettes à l'ARDRA (Amplified Ribosomal DNA Restriction Analysis) afin de regrouper et classer les différents fragments d'ADN en 686 OTUs (Operational Taxonomical Units). Ceux-ci ont alors été séquencés et analysés à l'aide de logiciels statistiques et bioinformatiques pour: (1) déterminer si …
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Epidemiología molecular en Escherichia coli procedente de fauna salvaje: resistencia antimicrobiana, virulencia y diversidad y diversidad genética
… factors. The third chapter focused on the analysis of distinct genetic elements involved in the selection, persistence and spread of resistance determinants among E. coli from different ecosystems (humans, domestic animals, wildlife, food). The first paper provides insights into the …
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Studium exprese mutantních alel a stavu X inaktivace ve vztahu ke klinickým projevům vybraných monogenních X vázaných onemocnění
… to examination. For the first time, the direct analysis of GLA gene transcript to detect the allele ratio was used alongside with the single-nucleotide polymorphisms in the IDS and LAMP genes for allele-specific expression (ASE) and the AR, RP2 and...
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ΜΕΛΕΤΗ ΤΩΝ ΦΥΣΙΚΩΝ ΠΛΑΣΜΙΔΙΩΝ ΤΟΥ ΣΤΕΛΕΧΟΥΣ ATCC 10988 ΤΟΥ ΑΙΘΑΝΟΛΟΠΑΡΑΓΩΓΟΥ ΒΑΚΤΗΡΙΟΥ ZYMOMONAS MOBILIS
… 1,85, 2,70, 16,7, AND 31,8 KB RESPECTIVELY. RESTRICTION MAPPING (PARTIAL) OF ALL THESE PLASMIDS WAS FOLLOWED AND THEIR HOMOLOGY LEVELS WERE DETERMINED. SIMILARLY, THEIR HOMOLOGY AGAINST TOTAL GENOMIC DNA AND PLASMID DNA OF Z. MOBILIS STRAINS ATCC 10988, NCIB 11163 AND CP4 WAS EXAMINED. …
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