Human intestinal microbiota and metabolites they produce in relation to host health

The aim of this thesis was to identify selected potential probiotic characteristics of Bifidobacterium longum strains isolated from human sources, and to examine these characteristics in detail using genomic and phenotypic techniques. One strain in particular Bifidobacterium longum DPC 6315 was the main focus of the thesis and this strain was used in both the manufacture of yoghurt and an animal study. In total, 38 B. longum strains, obtained from infants and adults, were assessed in vitro for the selected probiotic traits using a combined phenotypic and molecular approach. Differentiation of the 38 strains using amplified ribosomal DNA restriction analysis (ARDRA) into subspecies indicated that of the 38 bifidobacterial strains tested, 34 were designated B. longum subsp. longum and four B. longum subsp. infantis.

Grantor dc:publisher

University College Cork

Year dc:date.issued

2014

Author dc:creator

• Russell, David A.

Advisors dc:contributor.advisor

• Fitzgerald, Gerald F.
• Stanton, Catherine
• Ross, R. Paul

• Bifidobacteria
• Probiotic
• Metabolic activities
• Human health
• Food applications

Statement dc:rights

• © 2014, David Russell

Licence dc:rights.uri

• http://creativecommons.org/licenses/by-nc-nd/3.0/

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10468/1761

OAI identifier oai:identifier

oai:cora.ucc.ie:10468/1761