Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"Multiplex PCR"”.
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Modeling dengue viral infection, insect transmission and multiplex PCR in humanized mice
… and vector based viral intervention. Lastly, a multiplex quantitative real time PCR (qRT-PCR) assay capable of detecting and differentiating all four dengue viral serotypes was also established. This assay was able to detect each of the viral serotypes in a single reaction allowing for detection …
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Exploring Multiplex PCR Assay for Identification of Enteric Parasites in High-Volume Reference Laboratories
… Currently, several companies have developed multiplex molecular PCR based assays to detect the most common enteric parasites (<em>Giardia, Cryptosporidium & Entamoeba histolytica</em>) in one sample within few hours. The aim of this review is to explore different FDA approved multiplexes, …
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Use of a Novel Combination of Multiplex PCR and DNA Barcoding in Assessing Authenticity of Ginseng Products
… of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing either Panax ginseng, Panax quinquefolius, or Panax notoginseng were obtained from an online vendor. Two composite samples per product …
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Genetic diversity in Neospora caninum
… different isolates of this parasite. A prototype multiplex PCR assay was developed for strain typing of N. caninum based on the length polymorphisms detected amongst three different repetitive markers two- minisatellites and one microsatellite. This method is simple, rapid, highly informative and …
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Identification of methicillin-resistant Staphylococcus aureus in horses using conventional and molecular techniques
… Staphylococcus aureus detection based on a PCR reaction is commonly used and various types of PCR-based assays were developed to assist in early detection of MRSA. The main aim of the study was to compare the currently used conventional microbiological techniques with a published multiplex …
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Prevalence of the related treponematoses syphilis and yaws and co-occurrence of pathogens causing similar skin lesions in Ghana
… test was used to determine their seroprevalence. Multiplex PCR and 16S rRNA gene sequencing were used to identify other microorganisms in these lesions. Additionally, 16S rRNA gene sequencing was used to compare the microbiota of yaws-like lesions with that of syphilis-like lesions. The results …
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Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction
… 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all …
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Detection of Giardia lamblia and Cryptosporidium parvum in the eastern oyster (Crassostrea virginica) harvested from the Great Bay Estuary
… goal of this study was to develop a multiplex polymerase chain reaction for the simultaneous detection of Giardia lamblia and Cryptosporidium parvum in oysters. An immunomagnetic separation technique was utilized to capture the Giardia cysts and Cryptosporidium oocysts from oyster …
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Entwicklung von Microarrays für die Multiparameteranalytik und Etablierung einer Multiplex-OnChip-PCR
… der DNA-Analytik ist derzeit die Realtime-PCR. Dieser Technologie sind hinsichtlich der Multiplexfähigkeit technologischen Hürden gesetzt, da derzeit nur eine Analyse von maximal vier Parametern parallel in einem Versuchsansatz erfolgen kann. Microarrays stellen hingegen die benötigten …
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Molekulare Untersuchungen zu Virulenzfaktoren und Klonalität enterohämorrhagischer Escherichia coli der Serogruppe O103
… erforderlich, mittels differenzierter Multiplex-PCR-Methoden können dabei auch Kombinationen relevanter Pathogenitätsfaktoren in einem Arbeitsgang erfasst werden. EHEC O103 traten mehrere Jahre nach E. coli O157:H7 als HUS-Erreger in Erscheinung und haben wahrscheinlich die dazu …
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Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli
… able to screen samples for STEC in a rapid and multiplexed format. Multiplex oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based …
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Detection and Identification of Gastrointestinal Pathogens in Clinical Samples: Comparison Between two Laboratory Developed Multiplex Real-Time PCR Platforms
… specificity and sensitivity. In recent years, multiplex molecular testing has made its way into the clinical laboratory for detection and identification of enteric pathogens directly from clinical stool samples. Multiplex molecular assays are able to identify several gastrointestinal pathogens …
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Differential diagnosis of tick-borne diseases and population genetic analysis of Babesia bovis and Babesia bigemina
… disease control strategies. In this study PCR assays based on the 18S and 16S rRNA gene sequences, that could identify Theileria / Babesia and Anaplasma / Ehrlichia pathogens of cattle respectively, were developed. In addition, PCR assays based on the β-tubulin gene that could detect T. …
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Novel molecular markers of disease-association among strains of Streptococcus suis: a genomic approach
… on to describe the design and evaluation of a multiplex-PCR tool targeting the three newly defined "pathotyping markers" in comparison to existing methods used to characterise S. suis. These findings were taken further by using the pathotyping markers to screen material scrapped from the …
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Avaliação do potencial alergênico do ácaro de poeira doméstica Glycycometus malaysiensis
… allergenic extracts were identified using the multiplex PCR technique and characterized using SDS-PAGE and Western Blot. A total of 57 sera from atopic patients had IgE reactivity to these extracts assessed by indirect ELISA. Results: Using Western blots without inhibition to the extract of D. …
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Etablierung einer Fluoreszenz-Multiplex-Polymerase-Ketten-Reaktion zur semiquantitativen und qualitativen Analyse des V H-Repertoires in humanen B-Lymphozyten
Mit der VH-Fluoreszenz-Multiplex-PCR steht ein neuartiges Screeningverfahren zur semiquantitativen und qualitativen Analyse der VH-Familienverteilung der schweren Kette des Immunglobulinmoleküls in humanen B-Lymphozyten zur Verfügung. In zwei Multiplex-PCRs werden IgH Produkte der sechs häufigsten …
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Comparison of prevalence of avian pathogenic Escherichia coli in South African and US poultry
… SA and USA. The extracted DNA was screened by a multiplex PCR for five APEC VAGs (cvaC, iss, iucC, tsh and irp2). The pathogenicity of each isolate was determined by comparing the number of genes detected in each isolate to a positive control. Isolates with two or more virulence genes were …
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… genotype of Ds only plants was confirmed through multiplex PCR and the location of insertions within the genome was determined through TAIL-PCR. Resulting product sequences were blasted against the pre-publication tomato genome browser to determine insertion sites. A population of 309 independent …
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