Detection and Identification of Gastrointestinal Pathogens in Clinical Samples: Comparison Between two Laboratory Developed Multiplex Real-Time PCR Platforms

<p>Infectious gastroenteritis is caused by a wide range of enteric bacteria pathogens including <em>Salmonella, Shigella, Campylobacter jejuni, </em>and <em> E. coli </em>0157. Conventional methods used in the detection of such pathogens including, culture, microscopy, biochemical identification, and serological testing, are labor intensive, time-consuming, and lack specificity and sensitivity. In recent years, multiplex molecular testing has made its way into the clinical laboratory for detection and identification of enteric pathogens directly from clinical stool samples. Multiplex molecular assays are able to identify several gastrointestinal pathogens in as little as a few hours. This paper compares the test methodology, workflow, turnaround time, and cost-effectiveness of two laboratory developed multiplex PCR assays referred to as Platform A and Platform B used in the detection and identification of six enteric pathogens. This analysis comparing the current (Platform A) to newly proposed (Platform B), found the implementation of platform B into our current workflow to be advantageous. The implementation of platform B will significantly reduce the analysis to reporting turnaround time by 5 hours. A reduction in turnaround time will help clinicians diagnose gastroenteritis in a more accurate and timely manner. Reducing delay of treatment, inconsistent antimicrobial therapy, and/or expensive medical care.</p> <h1> </h1>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Master's Thesis

Discipline thesis:degree_discipline

Clinical Laboratory Sciences

Year dc:date.available

2018

Author dc:creator

• Garibay, Geybi Ariana

Contributors dc:contributor

• LaRonda S. Frazier
• Maria C. DeSousa, JD, MPA, CLS

• Gastrointestinal Pathogens
• Bacteria
• Equipment and Supplies

Repository record dc:identifier

https://scholar.dominican.edu/masters-theses/321

OAI identifier oai:identifier

oai:scholar.dominican.edu:masters-theses-1339