Exploring Multiplex PCR Assay for Identification of Enteric Parasites in High-Volume Reference Laboratories

<p>The conventional methods used for the identification of enteric parasites are microscopy (O&P) and ELSA. The O&P method itself is subjective, laborious, time consuming, lacks sensitivity, and specificity. The ELISA assays are also labor intensive and require multiple sample preparations. High-volume reference laboratories process hundreds of specimens for O&P and ELISA daily. Currently, several companies have developed multiplex molecular PCR based assays to detect the most common enteric parasites (<em>Giardia, Cryptosporidium & Entamoeba histolytica</em>) in one sample within few hours. The aim of this review is to explore different FDA approved multiplexes, compare them with the conventional method, and suggest the best fitted multiplex molecular test to be utilized in high-volume reference laboratories. In comparison to the conventional method, multiplex PCR assays are expensive; however, high-volume reference laboratories might justify the cost by consolidating different test orders and utilizing one specific panel based on the patients’ population.</p>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Culminating Project

Discipline thesis:degree_discipline

Clinical Laboratory Sciences

Year dc:date.available

2019

Author dc:creator

• Forghani Sardaghi, Narghes

Contributors dc:contributor

• Howard Koo, MS, CLS
• LaRonda S. Frazier, MS

• Giardia
• cryptosporidium
• Entamoeba histolytica
• Investigative Techniques

Repository record dc:identifier

https://scholar.dominican.edu/clinical-laboratory-sciences-culminating-projects/6

OAI identifier oai:identifier

oai:scholar.dominican.edu:clinical-laboratory-sciences-culminating-projects-1009