Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"Ionomycin"”.
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Binding and transport of calcium by synthetic analogues of ionomycin
The syntheses of simple analogues of ionomycin (3), namely a series of f3-diketoco-carboxylic acids 4-6, 28-30 and 33-35, were achieved by consecutive alkylation of the dianion of 2,4-pentanedione (7) with appropriate bromides and subsequent oxidation of theP-diketo tau-alcohols. The transport of …
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Abnormal regulation of T lymphocytes from aged rats contributes to immunosenescence
… lectin Con A, the calcium ionophores A23187 and ionomycin and the protein kinase C activator, PMA. Similar to human T lymphocytes, but unlike mouse lymphocytes, rat splenocytes and T lymphocytes proliferate in response to calcium ionophores in the absence of detectable IL 2 or IL 4. Proliferation …
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Sevofluran hemmt die DNA-Bindung des Transkriptionsfaktors Aktivator Protein-1 und induziert Apoptose in T-Lymphozyten
… NF-kappaB, SP-1 und <br>Oct-1 wurde mit PMA und Ionomycin induziert. Im EMSA konnte erstmals eine <br>zeit- und dosisabhängige Hemmung der DNA-Bindungsaktivität des <br>Transkriptionsfaktors AP-1 nach Inkubation mit Sevofluran nachgewiesen <br>werden. Kompetitionsansätze zeigten eine …
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FHL2 Inhibits Calcineurin and Represses Pathological Cardiac Hypertrophy
… active calcineurin or the calcium ionophore, ionomycin. Importantly, activation of the RCAN1 promoter by ionomycin, in control and FHL2 knockdown cells, was abolished by the calcineurin inhibitor cyclosporin A, confirming the calcineurin dependence of the response. Over-expression of FHL2 in …
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Magnesium Regulation of Calcium in Essential Hypertension
… accumulation in response to a five minute 10 nM ionomycin challenge was estimated in blood mononuclear cells (MNC) loaded with the calcium indicator fura-2 AM. Plasma and RBC total magnesium were measured and found to be considerably lower in magnesium-deficient rats. Magnesium-deficient RBC of …
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Calcium-dependent changes of apical membrane sodium channel density and open probability in frog skin
… to investigate whether treatment of tissues with ionomycin, quinine, or acidification of basolateral solution shared similar mechanisms of inhibition of Na$\sp{+}$ absorption. Experiments were done using staircase protocols (SC) that allowed evaluation of the dependence on blocker concentration of …
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Regulation of STAT6 and STAT4 Phosphorlation by Histamine
… histamine followed by stimulation with PMA plus ionomycin and/or IL-12 and IL-4. The levels of phosphorylation and unphosphorylated STAT6 and STAT4 were assessed by immunoblot technique. Histamine caused upregulation of phosphorylation of STAT6 and STAT4 when stimulated with PMA + ionomycin. HI …
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Methylation Change of Histone H3 and CpG Sites at the TIM-3 Gene Promoter in Response to Stimulation
… 그러나 phorbol 12-myristate 13-acetate (PMA)와 ionomycin (A23187) 자극에 의한 T 세포 활성화에 따른 DNA 메틸화 변화는 일어나지 않았다. 다음으로 TIM-3 promoter에서의 히스톤 H3 메틸화는 PBA와 A23187 자극에 의한 T 세포를 활성화 시켰을 때 proximal TIM-3 promoter (-954 ~ -34) 부위에서 H3K4me2의 수준이 증가되었고 distal TIM-3 promoter (-1549 ~ -1048) 부위에서 H3K9me3와 …
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K+-Induced Smooth Muscle Calcium Sensitization Requires RhoA Kinase (ROK) Translocation to Caveolae Which Is Inhibited in Non-Neuronal Cell Memory
… artery. Y-27632 also inhibited Bay K-8644- and ionomycin-induced MLC phosphorylation and force, but did not inhibit KCl-induced calcium entry or peak (~15”) force. Moreover, KC1 and Bay K-8644 nearly doubled the amount o f ROK colocalized to caveolae at 30”, a time that preceded inhibition of …
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Untersuchung der passiven Kernporenpermeabilität mit Fluoreszenzfarbstoffen in HT29- und HeLa-Zellen
… dabei entweder mit Agonisten oder dem Ionophor Ionomycin entleert. Auch ein möglicher Einfluß einer dauerhaft erhöhten [Ca2+]i wurde untersucht. Die in dieser Arbeit vorgestellten Experimente zeigen, daß die Entleerung der Calcium-Speicher keinen Einfluß auf die Kernporendurchlässigkeit hatte. …
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Characterization of the Tetraspanin protein Tspan18
… mimicked the effect of the calcium ionophore ionomycin. For the secondary thesis aim, over-expression of Tspan18 or other tetraspanins did not affect signalling by the platelet collagen receptor GPVI. Finally, Tspan18 mRNA was found to be relatively highly expressed in endothelial cells and …
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Innate Lymphoid Cell Diversity in Pregnancy and Ovarian Cancer
… respond poorly to ‘missing self’ and PMA plus ionomycin treatment. However, upon cross-linking of an activating KIR they can degranulate and produce cytokines/chemokines. dNK1 responsiveness to activating KIR increases with the expression of additional KIR which may be a result of increased …
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Staphylococcal Enterotoxin B Induced Clonal Anergy in αβ T Cell Receptor Transgenic Mice
… SEB. The anergic cells could respond to PMA and ionomycin suggesting that a proximal signal transduction step was affected. Cells from transgenic mice primed with OVA peptide, the specific antigen, were not anergic. Thus, in this system, the ability to tolerize mature T cells appears to be a …
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A novel method of generating Dendritic cells in vitro using the KG-1 cell line and its use as a model for testing effects of lactic acid bacteria
… cells stimulated with 10 ng/ml PMA and 100 ng/ml Ionomycin were found to be the ideal model for obtaining Dendritic Like Cells (DLCs) in vitro. The effect of lactic acid bacteria on KG-1 differentiation was also tested using two immunomodulatory strains, Lactobacillus rhamnosus R0011 and …
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Glia-neuron signaling
… the release of EAAs from glia. The addition of ionomycin caused a calcium-dependent release of EAAs. Taken together, these data demonstrate that calcium is both necessary and sufficient for stimulating the release of EAAs from DRG glia;Bradykinin was applied to mixed neuron-glial cultures …
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The use of different blood sample preparations in the development of biomarkers for the environmental monitoring of domestic farm animals
… with phorbol 12-myrisate 13-acetate and ionomycin (PMA+I). These results show that frozen blood gives comparable responses to fresh blood samples in the toxicological and immune assays used.
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Effect of phosphorylcholine-based small molecule analogues of the immunomodulatory nematode product ES-62 on mast cell function
… by BMMC activated by cross-linking bound IgE or ionomycin/PMA. Finally, immunofluorescent staining showed that SMAs 63 and 64 and in addition 39, induced the global degradation of PKC-α from both the cytoplasm and the plasma membrane. It can be assumed from these date, that these SMAs …
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Single-cell Functional Profiling of Lymphocytes for Cancer Immunotherapy
… by phorbol 12-myristate 13-acetate (PMA) and ionomycin (< 3 h), and no evidence of cooperation between NK cells to synergistic activation or faster IFN-γ secretion. These results establish our technology as an investigational tool for cellular phenotyping and real-time protein secretion of …
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Intrinsic heterogeneity in the survival and proliferation capacities of naïve CD8⁺ T cells
… of the TCR, pathway (same dose of PMA/ionomycin) as compared to CD51' cells. In contrast, CD510 cells survive better in conditions of cytokine deprivation. The selective proliferation in response to IL7 as well as the relative CD5 level is preserved even after several rounds of …
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Regulation der BOB.1/OBF.1-Expression und der BOB.1/OBF.1-Proteinstabilität
… BOB.1/OBF.1 erst nach Stimulation mit PMA und Ionomycin. Um die unterschiedliche Regulation in B-Zellen versus T-Zellen zu untersuchen wurde der BOB.1/OBF.1-Promotor kloniert. Die Analyse der Promotor-Sequenz ergab Bindungsstellen für die Transkriptionsfaktoren CREB, NF-AT und ein SRY-Motiv. In …
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