Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 25 for “"GST pull-down"”.
-
Distribution and targeting of CLC-3
… at later times to localize in large vesicles. GST pull-down assays demonstrated that the N-terminal of ClC-3 binds to both clathrin and AP-2. Deletion of dileucine cluster within the cytosolic N terminal (amino acids 13-19) resulted in a molecule that had decreased endocytosis and increased …
-
Screening auf Interaktionspartner für den hämatologischen Rezeptor PRV-1 : PRV-1 interagiert mit Decorin, C1qB und Defensin alpha 3
… exprimiertem Protein durchgeführter GST Pull Down Assay keine konsistenten Ergebnisse lieferte, wurde ein bakterieller GST Pull Down Assay etabliert. Darüber konnte für die Proteine Decorin, C1qB und Defensin in vitro eine physikalische Interaktion mit PRV-1 nachgewiesen werden. …
-
Identification of binding protein with kinesin18B
… with kinesin18B using a yeast two-hybrid system, GST pull-down assay, immunoprecipitation. This protein is Microtubule-associated protein RP/EB family member 1 containing a calponin homology(CH) domain at their N terminus and an EB1-like C-terminal motif at their C terminus : the functional …
-
The regulation of the CAMP signalling pathway in the human pathogenic fungus, Paracoccidioides brasiliensis
… receptors (GPCR). Adenylyl cyclase acts downstream of these G proteins. Get subunits are required to regulate the activity of adenylyl cyclase (AC), which controls the level of cellular cAMP (lvey and Hoffman, 2005). Protein Kinase A (PKA), which is activated by cAMP, is required for …
-
Suche nach Interaktionspartnern mit dem ATP-abhängigen Kaliumkanal der Niere, ROMK, durch "Yeast-Two-Hybrid-Screening"
… 13 Proteine zur weiteren Abklärung übrig. GST-Pulldown-Experimente und Immunfluoreszenz brachten weitere Aufschlüsse und Erkenntnisse zur Interaktion zwischen ROMK und seinen Partnern. Folgende Erkenntnisse konnten aus den Versuchen gewonnen werden: *) 174 positive Klone interagierten bei …
-
Investigating Potential Multi-Protein Complexes Involving Emx2
… a yeast two-hybrid screen and then confirmed by pull-down assays. The goal of this project is to use GST pull-down assays determine whether or not various combinations of these five proteins can interact to form a larger complex. The results indicate that larger protein complexes can be formed …
-
Untersuchung von Proteinkomponenten der ER-Golgi Recycling-Maschinerie von Hefe
… zwischen Acc1p und Sec22p konnte sowohl mit GST- pull-down"-Experimenten in vitro als auch durch Kreuzungen von Mutantenstämmen genetisch bestätigt werden. Untersuchungen weiterer SNAREs ergaben, daß auch Ufe1p und Sed5p sowohl im Zwei-Hybrid-System als auch GST- pull-down"-Experimenten an …
-
Functional Characterization of Amphiphysin in Drosophila melanogaster
… investigate novel protein partners for Amph. A GST pull-down approach identified actin as a potential Amph partner, consistent with studies in yeast. However, I could not confirm a direct interaction between Amph and actin in Drosophila. Another candidate partner was the actin-nucleating …
-
Targeting Traf6 For Cancer Therapeutical Development
… identified as hits were verified using a GST-pull down assay, comparing inhibition to a RANK decoy peptide. Since many drugs fail due to lack of efficacy and toxicity, predictive models for the evaluation of the LD50 and bioavailability of our TRAF6 hits, and these models can be used …
-
Identifizierung und Charakterisierung von IPOD, einem neuen Interaktionspartner der DNA-Methyltransferase Dnmt2 aus Drosophila melanogaster
… konnte in Co-Immunopräzipitations-Assays und GST Pull-down Assays bestätigt werden. Dabei gelang der Nachweis, dass die N-terminale Domäne von Dnmt2 für die Wechselwirkung mit IPOD verantwortlich ist. In Übereinstimmung mit der Konservierung von Dnmt2 in Drosophiliden zeigte eine nähere …
-
Isolierung und Charakterisierung von Organisationsfaktoren des Vimentin-Filamentsystems
… Chaperon aus der Hsp40-Proteinfamilie. Durch „GST-pull-down“-Experimente mit der Vimentin-Rod-Domäne und Präzipitationsversuchen mit assemblierten Vimentinfilamenten konnte das Hsp40-Protein aus einem HeLa-Zell-Lysat isoliert werden und somit eine biochemische Interaktion zwischen beiden …
-
Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… we used a modified Glutathione S-transferase (GST) pull down assay to investigate the role of the ERE in P160 coactivator recruitment. In the absence of the cERE, addition of whole cell extract caused a 3-4 fold reduction in ER-coactivator interaction, compared to binding observed without …
-
S100A4 regulation and function
… of actin cytoskeleton reorganization. Using GST pull-down and immunoprecipitation assays, I demonstrate that Rhotekin is a direct and specific partner of S100A4. Confocal microscopy showed that S100A4 co-localizes with Rhotekin. Using RNAi, I found that suppression of both S100A4 and Rhotekin …
-
Identifizierung und Charakterisierung von Interaktionen der Nichtstrukturproteine NS1 und NS2 des Respiratorischen Synzytialvirus mit Proteinen der Wirtszelle
… and p68 with both NS-Proteins was also shown in GST pull down assay in vitro. Engineered BRSV encoding a truncated p68 showed accelerated replication in MDBK and Vero cells, whereas growth of NS1/NS2 deletion mutants expressing the truncated p68 was unaffected. This indicates that the presence of …
-
Characterisation of LITAF, a protein associated with Charcot-Marie-Tooth disease type 1C
… partners were screened using the traditional GST pull-down assay and the in-situ proximity labelling assay, BioID. A number of novel potential binding partners were identified in both assays. Among the list of potential binding partners, BAG3 was captured in both pull-down assays and was …
-
Part I: Steroid Receptor CO-Activator 3 (SRC-3) Expression in Lung Cancer and its Role in Regulating Cancer Cell Survival and Proliferation; Part II: Development of Phosphospecific Peptoid Ligand
… phenotype of these cancers. siRNA-mediated down-regulation of SRC-3 in high-expressing tumor cells significantly inhibited tumor cell growth and induced apoptosis. The effect of SRC-3 down-regulation on cell phenotypes correlated with a cell line's endogenous expression level of the gene. …
-
Developmental trajectory of synaptic vesicle recycling in wild type and neurodevelopmental disorder mqodels
… be the next key question to address. Through GST pull-down assay and mass spectrometry, I have identified Dynamin-1, a GTPase that constricts and fissions SVs during endocytosis, as a potential novel interacting partner of FMRP, opening up a new avenue for studying SV recycling in Fmr1 KO …
-
The mechanism of HIV-1 Nef-mediated downregulation of CD4
… cells. Early studies suggested that Nef induces downregulation by linking the cytosolic tail of CD4 to components of the host-cell protein-trafficking machinery. However, the specific sorting pathway that Nef uses to modulate CD4 expression remained uncertain. According to one model, Nef was …
-
G-Protein gekoppelte Rezeptoren für Parathormon : molekulare Determinanten der intrazellulären Signalwegankopplung
… von Koimmunpräzipitations-Experimenten und von GST-pull-down-Assays die Interaktion von PDZK1 mit dem P1R zu verifizieren. PDZK1 bindet an eine Liganden-Bindungsdomäne innerhalb des C-terminalen Abschnitts des P1R, vermutlich an die letzten vier Aminosäuren. Durch einen Hefe-Interaktionstest …
-
Cell Biology of the ICA69 protein family in Neurosecretory cells
… with Rab2 from INS-1 cells extracts. GST-pull down assays demonstrated that this interaction is GTP-dependent. Furthermore, confocal microscopy indicated that ICA69 and Rab2 extensively colocalize in particulate structures throughout the cytoplasm. Immunocytochemistry and subcellular …
Page 1 of 2