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Showing 1 to 20 of 22 for “"GAL4/UAS"”.

  1. Lokalisierung eines Gedächtnisses bei Drosophila melanogaster

    Fischer, MatthiasUniversität Würzburg

    … rutabaga in rut-mutanten Fliegen mit Hilfe des Gal4/UAS-Systems ausschließlich in den Pilzkörpern, bzw., im Gegenexperiment, nur außerhalb der Pilzkörper zur Expression gebracht. rut kodiert für die Adenylatcyclase I, die mit synaptischer Plastizität bei Drosophila, Aplysia und Mäusen in …

    wurz-thes Repository record

  2. Spatial Expression Patterns of theSnmp Gene Family In Drosophila Melanogaster

    Staples, Jon

    … pattern for SNMP1 as well as SNMP2. Via the GAL4-UAS transgenesis system in tandem with immunohistochemical methods, we show that SNMP1 expresses in neural and support cells of the antennae as is consistent with the aforementioned studies. However, it is also shown that SNMP1 expression is …

    south-carolina Repository record

  3. Genetic Circadian Mosaics and the Clock Network’s Contributions to Sleep

    Widziszewski, LukaszThe Graduate School and University Center of The City University of New York2022

    … reinstated the Period (PER) protein using the Gal4-UAS system in specific subsets of the circadian clock neuron network using the <em>per<sup>01</sup></em>mutant fly as the base. This mutant fly lacks the PER protein, a critical protein essential for driving the negative feedback loop which …

    cuny-grad Repository record

  4. A Neuroprotective Role for Mir-1017, a Non-Canonical Mirna

    de Cruz, Matthew2019

    … host transcript for miR-1017. We utilized the GAL4/UAS system, to observe the miR-1017 expression pattern; furthermore we witnessed a disrupted feedback loop in the miR-1017 null background resulting in higher Da2 transcriptional activation. This could be phenocopied with the acetylcholine …

    usm Repository record

  5. Role of Drosophila Rap/Fzr (Cdh1) in retinal axon targeting and its interactions with Loco, Liprin-a and Ras

    Gronska, Marta

    … Mosaic analyses experiments using FLP-FRT and GAL4-UAS techniques show that Rap/Fzr functions in a cell autonomous manner. To test for possible role of other signaling molecules and interactions with Rap/Fzr, we have examined rap phenotypes in double mutant combinations with Loco, Liprin-α, …

    cuny Repository record

  6. Characterization of Codon Optimized Wild Type TDP-43 Mediated Neurodegeneration in a Drosophila Model for ALS.

    Yusuff, TanzeenThe University of Texas Medical Branch at Galveston2017

    … optimized wild type TDP-43 using the binary GAL4/UAS system, as well as direct promoter fusion constructs with glass promoter. Codon optimized TDP-43 misexpression causes robust phenotypes in the adult eye, wings and bristles in the notum. Compared to human transgenic flies, the codon …

    utmb Repository record

  7. Development of Tools to Assess the Effects of Lunasin on Normal Development and Tumor Progression in Drosophila Melanogaster

    Jones, Gillian E.

    … lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. Progeny were assessed for phenotypic alterations and …

    wku-diss Repository record

  8. Molekulare und funktionelle Charakterisierung der reggie/flotillin Gene in Drosophila melanogaster

    Höhne, Martin

    … wo diese zu Bündeln zusammengefasst werden. Mit GAL4/UAS-Fehlexpressionsstudien konnte die generelle Hypothese der Beteiligung an Adhäsionsprozessen weiter gestützt werden, da reggie-Fehlexpression während der Entwicklung des Komplexauges mit zelladhäsionsabhängigen Prozessen interferiert. <br>Im …

    freiburg-diss Repository record

  9. Regulation of cytochrome P450 genes in drosophila melanogaster by the chemical environment

    McDonnell, Cynthia M.University of Illinois at Urbana-Champaign2010

    … conserved across Drosophila species, by using a GAL4/UAS RNAi system to study the reproductive effects of knocking down the gene Cyp4g15. Using molecular modeling of the Cyp4g15 protein, I propose a potential substrate for this enzyme, the pheromone cis-vaccenyl acetate.

    uiuc Repository record

  10. Causes of Naive Ethanol Avoidance in Drosophila Melanogaster

    Ritz, Morgan Paige2020

    … To interfere with neuronal function, I used the Gal4/UAS system, which allows for tissue specific manipulation of the activity of both neurons and genes. RESULTS: On average, Drosophila flies ate less sucrose when ethanol was added. One reason for this was that fewer flies initiated feeding. Upon …

    utswmed Repository record

  11. Using <i>Drosophila melanogaster</i> as a Whole-Model Animal System to Elucidate the Mechanism of Action of Novel Anticancer Agents

    Jones, Amy R.University of Cincinnati2012

    … Mutants were generated using the Gal4-UAS system coupled with RNA interference. Knockdown was achieved for <i>Sod1</i>, a key gene in the antioxidant pathway, and <i>Ercc1</i>, a DNA repair gene. Drosophila were microinjected with the oxidatively agents, An-Hq and …

    ohiolink Repository record

  12. OMB and ORG-1

    Porsch, MatthiasUniversität Würzburg

    … of ectopic org-1 were analyzed using UAS-org-1 transgenic flies and a number of different Gal4 driver lines. Misexpression of org-1 during imaginal development interfered with the normal development of many organs and resulted in flies with a plethora of phenotypes. These include a …

    wurz-thes Repository record

  13. The role of drosophila protein kinase doubletime in circadian period determination, morning and evening oscillators and tauopathy

    Venkatesan, AnandakrishnanUniversity of Missouri--Kansas City2012

    In this dissertation, I used the GAL4-UAS binary expression method to overexpress mutant and wild type forms of the circadian protein kinase DBT in order to address several basic questions about DBT's biological functions. Different dbt mutations either shorten or lengthen the circadian period, …

    umkc Repository record

  14. Analysis of Tribolium head patterning by forward and reverse genetics and transgenic techniques

    Schinko, Johannes Benno2012

    … Ziel meiner Arbeit das binäre Expressionssystem GAL4/UAS für Tribolium zu etablieren. GAL4 wird durch Hitzeschock aktiviert, indem der Tribolium hsp68 promotor verwendet wird. Nach dem Hitzeschock wird das Reportergen über das UAS Konstrukt sowohl während allen Stadien der Triboliumentwicklung …

    goettingen Repository record

  15. Μελέτες σε μοντέλα Drosophila νευρο-εκφυλιστικών ασθενειών

    Κεντρωτή Ιωάννα, Kentroti Ioanna2026

    … γενετικό σύστημα στοχευμένης γονιδιακής έκφρασης GAL4-UAS. Η μελέτη πραγματοποιήθηκε στα κεφάλια ενήλικων εντόμων ηλικίας 7-10 ημερών. Παρατηρήσαμε επίσπευση φαινοτύπων της γήρανσης, όπως μεταβολές που υποδηλώνουν δυσρύθμιση στο μονοπάτι Keap1/CncC, καθώς και υποβάθμιση των μονοπατιών πρωτεόλυσης …

    athens Repository record

  16. Die transkriptionelle Regulation des roughest Gens in Drosophila melanogaster

    Apitz, Holger

    … identifiziert und durch die Konstruktion von rst-Gal4 Linien charakterisiert. Hierdurch kann gezeigt werden, dass das rst Gen durch eine komplexe Anordnung von Enhancer-Modulen reguliert wird. Unter anderem sind spezifische rst cis-regulatorische Sequenzen identifizierbar, die eine Expression in …

    freiburg-diss Repository record

  17. MUSCLE-SPECIFIC MECP2 MISEXPRESSION INDUCES SKELETAL AND VISCERAL MUSCLE DEFECTS RESCUED BY BUTYRATE SUPPLEMENTATION IN DROSOPHILA

    CONSONNI, GAIAUniversità degli Studi di Milano2026

    … wild-type or mutant human MECP2. Using the Gal4/UAS system, tissue-specific expression revealed that muscle-targeted MECP2 overexpression led to the strongest phenotypes, such as reduced viability, disorganized skeletal and visceral muscle, impaired locomotion, and delayed gut transit. In …

    milano Repository record

  18. Optische Visualisierung neuronaler Aktivität : Etablierung des in-vivo Calcium-Imaging mit dem genetisch codierten Sensor Yellow Cameleon 2.1 und Untersuchung der olfaktorischen Codierung im Gehirn von Drosophila melanogaster

    Spall, ThomasUniversität Würzburg

    … Cameleon 2.1 – Konstrukt wurde mittels der GAL4 – UAS – Technik in verschiedenen olfaktorischen Gehirnzentren exprimiert. Von besonderer Relevanz für die Experimente zur olfaktorischen Codierung war dabei die GAL4 – Treiberlinie GH146. Mit ihrer Hilfe konnte das Fusionsprotein in den …

    wurz-thes Repository record

  19. AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION

    Martinez Quiles, Maria LorenaOxford Brookes University2021

    … first approach, genetic assays exploiting the GAL4/UAS system were used to drive the tissue-specific RNAi downregulation of gro and genes involved in Pol II pausing in the developing wing and eye. These assays revealed that gro interacts genetically with the pausing factors Trl, Nelf, bin3, and …

    oxford-brookes Repository record

  20. Das Cysteine-String-Protein in Drosophila melanogaster: Molekulare und funktionelle Analyse verschiedener CSP-Mutanten; Ein Modell zur räumlich und zeitlich kontrollierten CSP-Expression

    Reisch, NatasaUniversität Würzburg

    … befasst sich mit dem Versuch, mithilfe des UAS/Gal4- und des Flippase/FRT -Systems die CSP-Expression räumlich und zeitlich zu kontrollieren. Dazu wurde aufgrund von Datenbankangaben eine minimale FRT-Sequenz aus Oligonukleotiden mit entsprechenden Linkern konstruiert. Das gesamte Csp-Gen …

    wurz-thes Repository record

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