Development of an Intelligent Heart-Cut Liquid Chromatography System for Chiral Analysis of Chemical Processes

Multi-dimensional HPLC techniques enable researchers to monitor multiple essential and sometimes, critical quality attributes (CQAs) in parallel. A unique three-column, four-dimension liquid chromatography system employing a two-loop heart-cut strategy is reported. The system is capable of re-circulating effluents from each dimension through a single detector and performing heart-cut operations using a programmable ten-port, two-position heart-cut valve. The first dimension of analysis measures peak ratios of products to reagents while the second dimension offers peak purity of the heart-cut material. The third dimension uses a chiral column to measure the enantiomeric excess (ee) of the chiral analyte and the effluent from the third dimension is re-analyzed via a fourth dimension to determine if any loss of chirality has occurred resulting from the analytical method itself. Performance of the setup was evaluated using the synthesis of Warfarin as a model chemical process.

Author dc:creator

• Bizzari, Nour Majid

Advisors dc:contributor.advisor

• Organ, Michael G.
• Pietro, William

• Organic chemistry

Statement dc:rights

• Author owns copyright, except where explicitly noted. Please contact the author directly with licensing requests.

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10315/34502

OAI identifier oai:identifier

oai:yorkspace.library.yorku.ca:10315/34502