Abstract
dc:description.abstractIn porcine ovaries, endothelin-1 (ET-1) may have an autocrine/paracrine role in follicular development. Endothelin-converting enzyme-1 (ECE-1) converts big endothelin-1 to ET-1. In situ hybridization was used to determine the ovarian compartment that synthesizes ECE-1. ECE-1 mRNA was detected in granulosa cells of large (10 mm) and medium (3 mm) antral follicles. This study, in conjunction with previous studies suggests a novel autocrine role of ET-1 as a possible local ovarian factor of follicular development. ET-1 may play a role during prostaglandin (PG) F2 alpha-induced luteal regression in the cow. Effects of PGF2 alpha treatment were examined during early and mid-luteal phases on ECE-1, ETA and ET-1 of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the mid-luteal phase CL.
Degree
thesis:*- Name thesis:degree_name
- MS
- Level thesis:degree_level
- Thesis
- Discipline thesis:degree_discipline
- Biology
- Year dc:date.available
- 2001
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Wright, Marietta Felicidad
Subjects
dc:subject × 2Identifiers
dc:identifier.*- Identifier
- https://researchrepository.wvu.edu/etd/1332
- OAI identifier oai:identifier
- oai:researchrepository.wvu.edu:etd-2335