{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-2335"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-2335","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Studies of the mammalian ovarian endothelin system","abstract":"In porcine ovaries, endothelin-1 (ET-1) may have an autocrine/paracrine role in follicular development. Endothelin-converting enzyme-1 (ECE-1) converts big endothelin-1 to ET-1. In situ hybridization was used to determine the ovarian compartment that synthesizes ECE-1. ECE-1 mRNA was detected in granulosa cells of large (10 mm) and medium (3 mm) antral follicles. This study, in conjunction with previous studies suggests a novel autocrine role of ET-1 as a possible local ovarian factor of follicular development. ET-1 may play a role during prostaglandin (PG) F2 alpha-induced luteal regression in the cow. Effects of PGF2 alpha treatment were examined during early and mid-luteal phases on ECE-1, ETA and ET-1 of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the mid-luteal phase CL.","abstract_html":"In porcine ovaries, endothelin-1 (ET-1) may have an autocrine/paracrine role in follicular development. Endothelin-converting enzyme-1 (ECE-1) converts big endothelin-1 to ET-1. In situ hybridization was used to determine the ovarian compartment that synthesizes ECE-1. ECE-1 mRNA was detected in granulosa cells of large (10 mm) and medium (3 mm) antral follicles. This study, in conjunction with previous studies suggests a novel autocrine role of ET-1 as a possible local ovarian factor of follicular development. ET-1 may play a role during prostaglandin (PG) F2 alpha-induced luteal regression in the cow. Effects of PGF2 alpha treatment were examined during early and mid-luteal phases on ECE-1, ETA and ET-1 of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the mid-luteal phase CL.","abstract_has_math":false,"creators":["Wright, Marietta Felicidad"],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001-12-01T08:00:00Z","date_published":"2001-12-01T08:00:00Z","updated_at":"2026-07-24T06:15:40Z","subjects":["Molecular biology","Cellular biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/1332"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/1332","href":"https://researchrepository.wvu.edu/etd/1332","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.1332","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Wright, Marietta Felicidad"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Molecular biology","Cellular biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.1332","https://researchrepository.wvu.edu/etd/1332"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In porcine ovaries, endothelin-1 (ET-1) may have an autocrine/paracrine role in follicular development. Endothelin-converting enzyme-1 (ECE-1) converts big endothelin-1 to ET-1. In situ hybridization was used to determine the ovarian compartment that synthesizes ECE-1. ECE-1 mRNA was detected in granulosa cells of large (10 mm) and medium (3 mm) antral follicles. This study, in conjunction with previous studies suggests a novel autocrine role of ET-1 as a possible local ovarian factor of follicular development. ET-1 may play a role during prostaglandin (PG) F2 alpha-induced luteal regression in the cow. Effects of PGF2 alpha treatment were examined during early and mid-luteal phases on ECE-1, ETA and ET-1 of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the mid-luteal phase CL."]},{"key":"dc:title","label":"Title","values":["Studies of the mammalian ovarian endothelin system"]}]}],"canonical_facts":{"dc:creator":["Wright, Marietta Felicidad"],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["In porcine ovaries, endothelin-1 (ET-1) may have an autocrine/paracrine role in follicular development. Endothelin-converting enzyme-1 (ECE-1) converts big endothelin-1 to ET-1. In situ hybridization was used to determine the ovarian compartment that synthesizes ECE-1. ECE-1 mRNA was detected in granulosa cells of large (10 mm) and medium (3 mm) antral follicles. This study, in conjunction with previous studies suggests a novel autocrine role of ET-1 as a possible local ovarian factor of follicular development. ET-1 may play a role during prostaglandin (PG) F2 alpha-induced luteal regression in the cow. Effects of PGF2 alpha treatment were examined during early and mid-luteal phases on ECE-1, ETA and ET-1 of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the mid-luteal phase CL."],"dc:identifier":["https://doi.org/10.33915/etd.1332","https://researchrepository.wvu.edu/etd/1332"],"dc:subject":["Molecular biology","Cellular biology"],"dc:title":["Studies of the mammalian ovarian endothelin system"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:15:40Z"}