Image Deconvolution Techniques for Single Molecule Studies

<p>One of the principal challenges in the field of biophysics, particularly that of protein-nucleic acid interactions, is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological systems. There is, nonetheless, a tradeoff between spatial and temporal resolution, or the ability to localize a molecule in space at increasingly shorter time scales. As such, this dissertation addresses these challenges that hinder single molecule studies by:: i) developing deconvolution techniques in order to localize both immobile and dynamic molecules from their single images with improved spatial and temporal resolution,: ii) determining a protein's diffusive properties with high temporal resolution, and: iii) applying our analytical methods to study model biological systems.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Year dc:date.available

2012

Author dc:creator

• DeSantis, Michael C.

Contributors dc:contributor

• Yan Mei Wang

Language dc:language

English (en)

DOI

https://doi.org/10.7936/K7ZS2TMM

OAI identifier oai:identifier

oai:openscholarship.wustl.edu:etd-1951