One of the principal challenges in the field of biophysics, particularly that of protein-nucleic acid interactions, is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological systems. There is, nonetheless, a tradeoff between spatial and temporal resolution, or the ability to localize a molecule in space at increasingly shorter time scales. As such, this dissertation addresses these challenges that hinder single molecule studies by:: i) developing deconvolution techniques in order to localize both immobile and dynamic molecules from their single images with improved spatial and temporal resolution,: ii) determining a protein's diffusive properties with high temporal resolution, and: iii) applying our analytical methods to study model biological systems.
","abstract_html":"<p>One of the principal challenges in the field of biophysics, particularly that of protein-nucleic acid interactions, is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological systems. There is, nonetheless, a tradeoff between spatial and temporal resolution, or the ability to localize a molecule in space at increasingly shorter time scales. As such, this dissertation addresses these challenges that hinder single molecule studies by:: i) developing deconvolution techniques in order to localize both immobile and dynamic molecules from their single images with improved spatial and temporal resolution,: ii) determining a protein's diffusive properties with high temporal resolution, and: iii) applying our analytical methods to study model biological systems.</p>","abstract_has_math":false,"creators":["DeSantis, Michael C."],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Yan Mei Wang"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-07-11T07:00:00Z","date_published":"2012-07-11T07:00:00Z","updated_at":"2026-07-24T06:13:23Z","subjects":[],"languages":["English (en)"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.7936/K7ZS2TMM"],"render_values":[{"text":"https://doi.org/10.7936/K7ZS2TMM","href":"https://doi.org/10.7936/K7ZS2TMM","code":true}]}]},"links":{"outbound_url":"https://openscholarship.wustl.edu/etd/951","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Yan Mei Wang"]},{"key":"dc:creator","label":"Author","values":["DeSantis, Michael C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-07-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English (en)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://openscholarship.wustl.edu/etd/951"]},{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.7936/K7ZS2TMM"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["One of the principal challenges in the field of biophysics, particularly that of protein-nucleic acid interactions, is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological systems. There is, nonetheless, a tradeoff between spatial and temporal resolution, or the ability to localize a molecule in space at increasingly shorter time scales. As such, this dissertation addresses these challenges that hinder single molecule studies by:: i) developing deconvolution techniques in order to localize both immobile and dynamic molecules from their single images with improved spatial and temporal resolution,: ii) determining a protein's diffusive properties with high temporal resolution, and: iii) applying our analytical methods to study model biological systems.
"]},{"key":"dc:title","label":"Title","values":["Image Deconvolution Techniques for Single Molecule Studies"]}]}],"canonical_facts":{"dc:contributor":["Yan Mei Wang"],"dc:creator":["DeSantis, Michael C."],"dc:date.available":["2013-07-09T07:00:00Z"],"dc:description.abstract":["One of the principal challenges in the field of biophysics, particularly that of protein-nucleic acid interactions, is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological systems. There is, nonetheless, a tradeoff between spatial and temporal resolution, or the ability to localize a molecule in space at increasingly shorter time scales. As such, this dissertation addresses these challenges that hinder single molecule studies by:: i) developing deconvolution techniques in order to localize both immobile and dynamic molecules from their single images with improved spatial and temporal resolution,: ii) determining a protein's diffusive properties with high temporal resolution, and: iii) applying our analytical methods to study model biological systems.
"],"dc:identifier":["https://openscholarship.wustl.edu/etd/951"],"dc:identifier.doi":["https://doi.org/10.7936/K7ZS2TMM"],"dc:language":["English (en)"],"dc:title":["Image Deconvolution Techniques for Single Molecule Studies"],"thesis:degree_discipline":["Physics"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T06:13:23Z"}