Design of Human Serum Albumin and Adenovirus Conjugation via Catcher/Tag Molecular Glue

<p>Adenovirus (Ad) has been the ideal cargo delivery mechanism, and its moderate immunological response makes it ideal for in vivo gene therapies since its discovery in 1953. However, the robust immunogenicity of the Ad capsid and low vaccine absorption via mucous membranes and epithelium put a limit on the process of developing intranasal vaccines. Efforts are being made to enhance the effectiveness of Ad vectors and numerous studies have demonstrated the remarkable capacity of human serum albumin (HSA) to extend plasma half-life and facilitate targeted intranasal delivery. In this study, we devised an innovative method for employing the Catcher/Tag molecular glue to shield Ad with HSA at a molecular precision level. The incorporation of Catcher/Tag technology provides us with a high degree of flexibility and precision in manipulating the construction process. This strategy circumvents the problem of re-designing and re-producing recombinant HSA-Ad vectors and allows us to select HSA shielding sites and proportions more efficiently.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biomedical Engineering

Year dc:date.available

2023

Author dc:creator

• Zhao, Peijie

Contributors dc:contributor

• David T. Curiel, Department of Radiation Oncology
• Michael Vahey, Jai Rudra

• Human Serum Albumin
• Adenovirus
• Conjugation
• Catcher/Tag Molecular Glue
• Biological Engineering
• Molecular, Cellular, and Tissue Engineering
• Other Biomedical Engineering and Bioengineering

Statement dc:rights

• I have not registered my thesis with the U.S. Copyright Office, but intend to later.

Language dc:language

English (en)

Identifier

https://openscholarship.wustl.edu/eng_etds/987

OAI identifier oai:identifier

oai:openscholarship.wustl.edu:eng_etds-2018