Transcriptomic Insights into the Morphological Variation Present in Bromeliaceae

<p>The Bromeliaceae family utilizes a wide range of adaptations to inhabit a variety of environments including dry ones. Many attribute the large adaptive radiation of Bromeliaceae throughout the Neotropics to three main features: absorptive trichomes, tank reservoirs, and CAM photosynthesis. Based on leaf morphology and arrangement, root type, and nutrient acquisition, Pittendrigh (1948) conservatively separated bromeliads into four main classes. These four main classes are designated Type I bromeliads, Type II bromeliads, Type III bromeliads and Type IV bromeliads. We used RNA-sequencing of leaf mRNA to investigate similarities and differences in gene expression which can be related back to the four distinct leaf morphologies in the Bromeliaceae family. We found several transcripts relating to the presence of a tank and absorptive trichomes. In addition, we found evidence of varying forms of carbohydrate synthesis for carbon storage during CAM photosynthesis. Lastly, transcriptomics differences indicate different drought survival strategies, with the most extreme differences occurring between Aechmea nudicaulis and Tillandsia gardneri. This study identified transcripts related to the morphological gradient and highlighted how each ecological type has a particular set of adaptations and strategies for survive in a particular regime.</p>

Name thesis:degree_name

Master of Science

Discipline thesis:degree_discipline

Department of Biology

Year

2015

Author dc:creator

• Gilkison, Victoria A.

Contributors dc:contributor

• Robert Wyatt (Director), Albert Meier, and Claire Rinehart

• RNA seq
• Drought
• gene expression
• Bromeliads
• Botany
• Plant Sciences

Repository record dc:identifier

https://digitalcommons.wku.edu/theses/1495

OAI identifier oai:identifier

oai:digitalcommons.wku.edu:theses-2486