CD133 Antibody Conjugation to Decellularized Human Heart Valves For Circulating Endothelial Progenitor Cell Capture

The long term efficacy of heart valve bioprostheses is limited by progressive degeneration characterized by immune mediated inflammation and calcification. To avoid this degeneration, decellularized heart valves with functionalized surfaces capable of rapid in vivo endothelialization have been developed. The aim of this study is to examine the capacity of CD133 antibody-conjugated valve tissue to capture circulating endothelial progenitor cells (EPCs). Decellularized human pulmonary valve tissue was conjugated with CD133 antibody at varying concentrations and exposed to CD133 expressing NTERA-2 cl.D1 (NT2) cells in a microflow chamber. The amount of CD133 antibody conjugated on the valve tissue surface and the number of NT2 cells captured in the presence of shear stress was measured. Both the amount of CD133 antibody conjugated to the valve leaflet surface and the number of adherent NT2 cells increased as the concentration of CD133 antibody present in the surface immobilization procedure increased. The data presented in this study support the hypothesis that the rate of CD133+ cell adhesion in the presence of shear stress to decellularized heart valve tissue functionalized by CD133 antibody conjugation increases as the quantity of CD133 antibody conjugated to the tissue surface increases.

Grantor dc:publisher

Wake Forest University

Year dc:date.issued

2015

Author dc:creator

• Vossler, John David

• CD133

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10339/57271

OAI identifier oai:identifier

oai:wakespace.lib.wfu.edu:10339/57271