The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana

Auxin transport polarity is established and maintained by the asymmetrical localization of auxin transport proteins in the plasma membrane. The <italic>SCD1<italic> (stomata cytokinesis defective) gene encodes a protein containing a DENN domain, which is predicted to function as a RAB-GEF, which may be important in membrane protein targeting. The <italic>scd1-1<italic> allele is a temperature sensitive mutant with a root phenotype evident at 25&deg; C, but not at 18&deg; C. The localization of auxin transport proteins, amount of auxin transport, and dependent physiological processes were examined in <italic>scd1-1<italic>. Basipetal or shootward auxin transport, and the dependent process of differential gravitropic growth, was lower in the <italic>scd1-1<italic> mutant than wild type at 25&deg; C, but not at 18&deg; C. Laser scanning confocal microscopy was used to examine the localization of auxin transport proteins PIN1, PIN2, PIN3, MDR1, and AUX1 fused to fluorescent proteins. The PIN protein reporters were expressed in fewer cells at lower intensity in <italic>scd1-1<italic> at 25&deg; C. PIN1 and PIN2 exhibited altered localization and accumulation in endomembrane bodies. The endomembranes that accumulated PIN2

Grantor dc:publisher

Wake Forest University

Year dc:date.issued

2011

Author dc:creator

• Isley, Jonathan Wayne

• auxin

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10339/33467

OAI identifier oai:identifier

oai:wakespace.lib.wfu.edu:10339/33467