The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans

Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.

Name dc:type.qualificationname

other

Level dc:type.qualificationlevel

rmaster

Grantor dc:publisher.institution

Victoria University of Technology

Year dc:date.issued

2004

Author dc:creator

• Leppik, James Alexander

• 0301 Analytical Chemistry
• 1106 Human Movement and Sports Science
• School of Sport and Exercise Science

Language dc:language

en