{"id":{"repo_id":"vu-aus","oai_identifier":"oai:eprints.vu.edu.au:17887"},"canonical_url":"https://search.dev.ndltd.org/etd/vu-aus/oai:eprints.vu.edu.au:17887","repository":{"repo_id":"vu-aus","name":"Victoria University (Australia)","base_url":"https://vuir.vu.edu.au/cgi/oai2"},"display":{"title":"The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans","abstract":"Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.","abstract_html":"Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.","abstract_has_math":false,"creators":["Leppik, James Alexander"],"institution":"Victoria University of Technology","degree_name":"other","degree_level":"rmaster","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-24T06:33:22Z","subjects":["0301 Analytical Chemistry","1106 Human Movement and Sports Science","School of Sport and Exercise Science"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Leppik, James Alexander"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2004"]},{"key":"dc:date.issued","label":"Date","values":["2004"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Human Movement, Performance and Recreation"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Victoria University of Technology"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://vuir.vu.edu.au/17887/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["rmaster"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["other"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["0301 Analytical Chemistry","1106 Human Movement and Sports Science","School of Sport and Exercise Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://vuir.vu.edu.au/17887/3/LEPPIK%20James-thesis_nosignature.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans"]}]}],"canonical_facts":{"dc:creator":["Leppik, James Alexander"],"dc:date":["2004"],"dc:date.issued":["2004"],"dc:description.abstract":["Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate."],"dc:format":["text"],"dc:identifier.uri":["https://vuir.vu.edu.au/17887/3/LEPPIK%20James-thesis_nosignature.pdf"],"dc:language":["en"],"dc:publisher.department":["Department of Human Movement, Performance and Recreation"],"dc:publisher.institution":["Victoria University of Technology"],"dc:relation.isreferencedby":["https://vuir.vu.edu.au/17887/"],"dc:subject":["0301 Analytical Chemistry","1106 Human Movement and Sports Science","School of Sport and Exercise Science"],"dc:title":["The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["rmaster"],"dc:type.qualificationname":["other"]},"updated_at":"2026-07-24T06:33:22Z"}