Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol

PARP-1 is a nuclear enzyme involved in a range of activities associated with DNA metabolism, playing a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop pharmaceutical reagents including cancer therapeutics. A major obstacle to this work however is that our knowledge of the relationship between structure and function of PARP-1 is rather limited. Structure - function studies of enzymes typically require the application of techniques for producing reasonable quantities of high quality protein. To this end, this thesis describes the development of a rapid and relatively simple approach to producing and purifying PARP-1.

Name dc:type.qualificationname

phd

Level dc:type.qualificationlevel

doctoral

Grantor dc:publisher.institution

Victoria University of Technology

Year dc:date.issued

2003

Author dc:creator

• Knight, Matthew

• 0601 Biochemistry and Cell Biology
• School of Engineering and Science
• 0304 Medicinal and Bimolecular Chemistry

Language dc:language

en