Back to results

Virginia Tech

Sources of Variation in the Microbiome of Pre-Weaned Dairy Calves

Abstract

dc:description.abstract

This study investigated the microbial colonization and maternal influences on the neonatal calf gut microbiome. Microbiome samples were collected from dams (n = 6) and calves (n = 6) using sterile flocked swabs. The vaginal, oral, and fecal bacterial communities were examined from the dam and the fecal community of calves was examined from birth to 60 d of age. Microbial communities varied by anatomical location and age of the calf. Metagenomic analysis 16s ribosomal DNA revealed ten phyla associated with microbiomes of the dam and the same ten phyla associated with calf feces at various time points: Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, Cyanobacteria, Verrucomicrobia, Spirochaetes, Tenericutes, Fibrobacteres, and Lentisphaerae. Overall, the calf meconium and fecal microbiome is influenced by a combination of the maternal vagina, oral, and fecal microbiomes. Further studies will be needed to identify the transference mechanisms of maternal microbes to offspring and the associated host-microbial interactions.

Degree

thesis:*
Name thesis:degree_name
Master of Science in Life Sciences
Level thesis:degree_level
masters
Discipline thesis:degree_discipline
Dairy Science
Department dc:contributor.department
Dairy Science
Grantor dc:publisher
Virginia Tech
Year dc:date.issued
2019

Author and committee

dc:creator, dc:contributor.*
Author dc:creator
  • Huffard, Haley Garrett
Chair dc:contributor.committeechair
  • Knowlton, Katharine F.
Committee members dc:contributor.committeemember
  • James, Robert E.
  • Cockrum, Rebecca R.

Subjects

dc:subject × 4

Rights

dc:rights
Statement dc:rights
  • In Copyright

Identifiers

dc:identifier.*
Dc Identifier Other
vt_gsexam:21757
OAI identifier oai:identifier
oai:vtechworks.lib.vt.edu:10919/93957

Chain of custody

source
Harvested from
Virginia Tech
Base URL
vtechworks.lib.vt.edu/oai/request
Last updated
2026-07-22
Source record
OAI-PMH GetRecord
citation

Huffard, Haley Garrett. Sources of Variation in the Microbiome of Pre-Weaned Dairy Calves. masters thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/93957