Modeling Nitrogen and Energy Metabolism in the Bovine

The objectives of this research were to: 1) evaluate the accuracy of the Molly cow model predictions of ruminal metabolism and nutrient digestion when simulating dairy and beef cattle diets, 2) advance representations of N recycling between blood and the gut and urinary N excretion in the model, 3) improve the representation of pH and to refit parameters related to ruminal metabolism and nutrient digestion in the model, 4) investigate how ruminal pH affects the microbial community, expression of carbohydrate-active enzyme transcripts (CAZymes), fiber degradation, and short chain fatty acid (SCFA) concentrations. To achieve the first objective, a total of 229 studies (n = 938 treatments) including dairy and beef cattle data, published from 1972 through 2016, were collected from the literature and used to assess the model accuracy and precision based on root mean squared errors (RMSE) and concordance correlation coefficients (CCC). Only slight mean and slope bias were exhibited for ruminal outflow of NDF, starch, lipid, total N, and non-ammonia N, and for fecal output of protein, NDF, lipid, and starch. However, ruminal pH was poorly simulated and contributed to problems in ruminal nutrient degradation and VFA production predictions. To achieve the second objective, representations including ruminal ammonia outflow, intestinal urea entry, microbial protein synthesis in the hindgut, and fecal urea N excretion, were added in the model. Total urea entry, gut urea entry, and urinary urea elimination rates collected from 15 published urea kinetics studies were used to derive related parameters. Significant improvements in predictions of variables describing ruminal N metabolism, blood urea metabolism and urinary N secretion were exhibited after the modifications. To achieve the third objective, a dataset assembled from the literature containing 284 peer reviewed studies with 1223 treatment means was used to derive parameter estimates for ruminal metabolism and nutrient digestions. After refitting the parameters, the model is even more robust in representing ruminal nutrient degradation compared to the initial model. Adding ammonia concentration as a driver to the pH equation increased the precision of predicted ruminal pH, and thereby, the precision of predicted VFA concentrations due to an improved representation of pH regulation of VFA production rates. To achieve the fourth objective, six cannulated Holstein heifers with an initial BW of 362 ± 22 kg (mean ± SD) were subjected to 2 treatments in a cross-over design. The treatments were 10 days of intraruminal infusions of both 1) distilled water (Control), and 2) a dilute blend of hydrochloric and phosphoric acids to achieve a pH reduction of 0.5 units (LpH). Statistical analyses indicated 19 bacterial genera and 4 protozoal genera were affected by low ruminal pH. We observed significant correlations between 54 microbes (43 bacterial and 11 protozoal genera) and 25 enzymes, of which 8 key enzymes participated in reactions leading to SCFA production, suggesting that the ruminal microbial community alters fiber catalysis and fermentation in response to altered pH through a shift in carbohydrate-active enzyme transcripts (CAZymes) expression. Overall, after the modifications and reparameterizations, 19.7 to 37.5% of RMSE with essentially no slope bias and minor mean bias were exhibited for of ruminal and fecal outflow of ADF, NDF, fat, and protein, suggesting the model is properly to represent nutrient degradation and digestion in the bovine. Considering ruminal microbes and CAZymes in predicting ruminal volatile fatty acid concentrations could explain more variance of observations.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Animal Sciences, Dairy

Department dc:contributor.department

Dairy Science

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2019

Author dc:creator

• Li, Mengmeng

Chair dc:contributor.committeechair

• Hanigan, Mark D.

Committee members dc:contributor.committeemember

• Guan, Leluo
• White, Robin R.
• Cockrum, Rebecca R.

• cattle
• microbes
• nutrient digestion
• rumen metabolism

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

vt_gsexam:18549

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/87090