Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum

vegetable crops. Micro-Tom is a model tomato cultivar used for research due to its small size and short time to fruiting. This project evaluated I. walleriana and Micro-Tom transformed with Agrobacterium. The construct contained GFP (green fluorescent protein) and hygromycin antibiotic-resistant selectable markers, and the antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence of transgenes was confirmed by PCR. Transgenic Impatiens was selfed for two generations. Transgenic Micro-Tom was selfed for 4 generations. Spinach was used as an INSV reservoir. Impatiens, spinach and Micro-Tom were mechanically inoculated with INSV and evaluated visually, with assay tests, ELISA testing, and PCR. Spinach was successfully infected with INSV six times of seven attempts. Impatiens and Micro-Tom had no successful inoculations of three and five attempts, respectively.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Horticulture

Department dc:contributor.department

Horticulture

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2018

Author dc:creator

• Sears, Vicki P.

• Impatiens walleriana
• Impatiens Necrotic Spot Virus
• INSV
• Solanum lycopersicum
• Micro-Tom
• Spinacia oleracea
• Spinach
• Orthotospovirus
• Tospoviridae
• Frankliniella occidentalis
• Western Flower Thrips
• transformation
• PCR

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

vt_gsexam:13772

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/81964