Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 3519 for “"PCR"”.
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The Effects of Known PCR Inhibitors on Droplet Digital PCR Performance
… that can inhibit the polymerase chain reaction (PCR). Inhibitors associated with forensic samples include substances that are endogenous with the sample (calcium, melanin), absorbed from the environment (humic acid, indigo dye)1, or introduced during laboratory processing (phenol1 , glove …
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Evaluation of direct PCR for forensic DNA profiling and the development of a direct PCR multiplex
The use of direct PCR with different types of sample was explored in this study. Genomic DNA preparations at various concentrations and buccal cell counts were deposited on commonly encountered substrates, recovered and amplified using direct PCR before subjecting them to capillary electrophoresis. …
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Real time PCR measurement by fluorescence anisotropy
Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues …
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Detection of enteroviruses in marine waters using RT-PCR.
… focused on (i) optimization of methods for RT-PCR detection of enteroviruses in marine waters, with particular attention to the issue of sensitivity vs inhibition (ii) comparison of enterovirus detection in marine waters by cell culture and RT-PCR and (iii) development of a method for detecting …
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Soybean transformation and analysis of transformed tissues using PCR
Factors which affect the transformation efficiency of Agrobacterium tumefaciens-mediated soybean transformation were studied. It was found that pre-induction of A. tumefaciens A281 using acetosyringone significantly increased the efficiency of transformation and this could be boosted further by …
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Simplified Low Copy Number Dna Analysis By Post Pcr Purification
… techniques. Here, various methods of post PCR purification were evaluated for their effects on the sensitivity of fluophore-based allelic detection. A method of post PCR purification is described which increases the sensitivity of standard 28 cycle PCR such that low copy number DNA …
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Oligonucleotide design and codon optimization for PCR-based gene synthesis
… from overlapping oligonucleotides by ligation or PCR; this thesis focuses on PCR-based methods. Many sets of oligonucleotides can be used to synthesize a given sequence; choosing the optimal set is a computational problem. A number of software tools for oligonucleotide design exist, but none are …
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Určování pohlaví kalouse ušatého (Asio otus) na základě PCR amplifikace DNA
… blood platelets in the bird quail and subsequent PCR reaction. This thesis also describes the PCR method, which is one of the most used methods in the todays laboratories of many different field of science. I also mention the individual components that are needed for the process of this reaction, …
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Mikroarray-basierte Detektion von mRNA aus Zellen mittels On-Chip-PCR
… werden können: erstens die On Chip- RT-PCR mit cDNA als Matrize und zweitens diese Methode als One-Step-Reaktion mit RNA als Matrize. Beide Methoden beruhen auf dem Prinzip der PCR an immobilisierten Primern auf einer Chipoberfläche. Diese Möglichkeit der exponentiellen Amplifikation ist …
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The development of PCR internal controls (PICs) for forensic DNA analysis
… amplification efficiency and the success of post-PCR processing. Chemicals associated with forensic samples can affect the amplification process while random errors occurring during pipetting and electrokinetic injection can cause variability. This can lead to either a reduced signal or lack of …
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… and require significant time and labor; λ-PCR, a new cloning method developed in the Senger lab, has a number of advantages compared to other cloning processes due to its employment of relatively inexpensive and widely available materials and time-efficiency. While the amount of lab work …
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Modeling dengue viral infection, insect transmission and multiplex PCR in humanized mice
… Lastly, a multiplex quantitative real time PCR (qRT-PCR) assay capable of detecting and differentiating all four dengue viral serotypes was also established. This assay was able to detect each of the viral serotypes in a single reaction allowing for detection of virus in mixed infection …
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The application of a PCR based species identification method to African wildlife
Molecular based species identification is a useful tool in forensic investigations as well as routine work. The ability to cheaply and quickly determine the species of origin of a sample has become increasingly necessary as incidents of wildlife poaching, illegal meat trade and the trade of …
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Towards the development of an RT-PCR protocol for malaria parasite genes
… was available, 3 '-RACE (a modified form of RT-PCR) was chosen as a method to obtain the gene sequence. Some of the obstacles encountered and the way that they were addressed are described. The synthesis of a representative, full-length, uncloned cDNA library of the AT-rich malaria genome was …
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Entwicklung von Microarrays für die Multiparameteranalytik und Etablierung einer Multiplex-OnChip-PCR
… der DNA-Analytik ist derzeit die Realtime-PCR. Dieser Technologie sind hinsichtlich der Multiplexfähigkeit technologischen Hürden gesetzt, da derzeit nur eine Analyse von maximal vier Parametern parallel in einem Versuchsansatz erfolgen kann. Microarrays stellen hingegen die benötigten …
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