Use of High Pressure Processing to Reduce Foodborne Pathogens in Coconut Water

Juices have been implicated in numerous foodborne outbreaks over the last couple of decades. The FDA requires a 5-log10 reduction in juice products, which is most commonly achieved through pasteurization. However, pasteurization deteriorates some sensorial properties and nutritive value. Coconut water (CW; classified as a juice), is rapidly gaining popularity increasing over 300% since 2005. CW has not been implicated in a microbial outbreak, but is thermally processed to achieve the required 5-log10 CFU/ml reduction, which results in negative organoleptic properties. The objectives of this study are to determine whether E. coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes can grow in CW, and evaluate the use of High Pressure Processing (HPP) to reduce populations of these bacteria in CW. The three pathogens were inoculated separately into CW and bacterial populations were enumerated over 24 hours. All three bacteria reached at least 8-log10 CFU/ml after 24 hours, which was not significantly different from the control (TSB). CW was then inoculated with each pathogen and processed using HPP (400, 5000, or 600 MPa) for 120 seconds. The D-glucose, D-fructose, sucrose, and phenol oxidase levels in the CW were assessed before and after treatments. Following processing, the pathogens were enumerated from the CW. All three pathogens were reduced by more than 6-log10 CFU/ml following treatments of 500 and 600 MPa, enough to achieve the mandatory 5-log CFu/m reduction. There were no significant changes in the D-glucose, D-fructose, sucrose, and phenol oxidase activity after any of the treatments.

Name thesis:degree_name

Master of Science in Life Sciences

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Food Science and Technology

Department dc:contributor.department

Food Science and Technology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2013

Author dc:creator

• Lukas, Anthony R.

Chair dc:contributor.committeechair

• Boyer, Renee R.

Committee members dc:contributor.committeemember

• O'Keefe, Sean F.
• Williams, Robert C.

• E. coli
• Salmonella
• Listeria
• Coconut Water
• High Pressure Processing

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

vt_gsexam:2016

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/24760