Inhibition of Bacillus subtilis respiration on glucose, formate and succinate by certain anesthetic agents

The non-proliferating or “resting” cell technic and the Werburg respirometer have been employed to study the respiration of <i>Bacillus subtilis</i> Koch-Novy with glucose, formate and succinate as substrates. The enzymatic oxidation of these substrates in the presence of cyanide, urethane, chloral hydrate, chloroform and other have been investigated and the effects of these agents over a range of concentrations have been noted. Graphs have been prepared for comparison of the oxygen consumption and carbon dioxide liberation values for <i>Bacillus subtilis</i> Koch-Novy in the presence and absence of the various inhibitory agents. Differences between the oxidation of succinate by bacteria and by mammalian brain tissue have been pointed out. Information concerning the oxidations of brain tissue was obtained from the previous work of Quastel et al and Grieg (11, 23, 24). Gaseous exchange which suggest some sort of metabolic action by <i>Bacillus subtilis</i> Koch-Novy on chloretone has been noted. The author has expressed an opinion regarding the advisability of employing members of the genus <i>Bacillus</i> for studies concerning the mode of section of anesthetic agents.

Name thesis:degree_name

M.S.

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Bacteriology

Department dc:contributor.department

Bacteriology

Grantor dc:publisher

Virginia Polytechnic Institute

Year dc:date.issued

1948

Author dc:creator

• Nutting, Leighton Adams

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10919/118857

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/118857