AFM-Assisted Nanofabrication using Self-Assembled Monolayers

This study describes the covalent and the electrostatic attachment of molecules, nano-particles, and proteins to patterned self-assembled monolayers. A scanning probe nanografting technique was employed to produce patterns of various sizes, down to 10 nm. Thus, we are able to demonstrate a degree of surface patterning which is an order of magnitude smaller than that used in the semiconductor industry. One efficient strategy for creating chemically specific nanostructures is to use the extraordinary catalytic properties of enzymes. However, as the dimension of a catalyst patch is reduced down to nanometer scale, it is difficult to detect the very low concentration of product. This study resolves the problem by developing a new strategy: the surface trapping of a product generated by a nanometer-scale patch of surface-bound enzyme. An array of proteins finds use when the array contains a number of different proteins. Toward this end, a new and convenient method for immobilizing enzymes is developed, which will allow the preparation of thin films containing several different catalytically-active enzymes on the nanoscale. The disadvantage of scanning probe nanografting technique is that the AFM tip loses resolution through wear during the patterning procedure. This study examines the possibility of developing a new AFM lithographic method to avoid wear: the use of enzymes covalently attached to a tip as a site-specific catalyst.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Chemistry

Department dc:contributor.department

Chemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2003

Author dc:creator

• Jang, Chang-Hyun

Chair dc:contributor.committeechair

• Ducker, William A.

Committee members dc:contributor.committeemember

• Anderson, Mark R.
• Esker, Alan R.
• Morris, John R.
• Tissue, Brian M.

• Atomic Force Microscopy
• Nanofabrication
• Self-Assembled Monolayer
• Lithography
• Acetylcholinestrase

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-01242004-013030

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/11103