Endogenous cyclic AMP-dependent phosphorylation in vitro of cytosolic proteins from Dictyostelium discoideum

Endogenous phosphorylation was measured in soluble fractions at four stages of Dictyostelium discoideum development. A peak of activity occurred at the slug stage, coincident with the appearance of cyclic AMP-dependent protein kinase. After partial purification by DE-52 cellulose and Sephacryl S-300 chromatography, cyclic AMP dependency of six cytosolic proteins was observed, with apparent subunit molecular weights of > 200,000, 110,000, 107,000, 91,000, 75,000, and 69,000. Phosphorylated bands at 107,000 and 91,000 MW were found to be specific for a partially-purified catalytic subunit prepared from the Dictyostelium holoenzyme form.

Name thesis:degree_name

M.S.

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Zoology

Department dc:contributor.department

Zoology

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1983

Author dc:creator

• Frame, Lynn Teresa

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10919/105979

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/105979