Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 66 for “"Holoenzyme"”.
-
RNA polymerase II holoenzyme components and regulation
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
-
Regulation of yeast RNA polymerase II holoenzyme transcription
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
-
Molecular genetic analysis of a yeast RNA polymerase II holoenzyme
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1994.
-
Functional antagonism of the RNA polymerase II holoenzyme by negative regulators
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
-
The B55α/PP2A Holoenzyme in Cell Cycle Exit, Maturation/Differentiation, and Cancer
… that functions as as a collection of trimeric holoenzymes. The trimeric PP2A holoenzyme is composed of the "A" scaffolding subunit, the "C" catalytic subunit, and a "B" regulatory subunit. The B subunit is the major determinant in substrate specificity and subcellular localization. Two …
-
Characterization and localization of a cyclic AMP dependant protein kinase from Dictyostelium discoideum
… and kinetic properties of the cAMP-dependent holoenzyme and its subunits. Gel filtration data suggests a holoenzyme Mr of 170,000-190,000, and catalytic and regulatory subunit Mrs of 40,000 and 49,000, respectively. These molecular weight determinations are compatible with an R₂C₂ subunit …
-
Structural Analysis of an RNA Polymerase Sigma Factor and Its Anti-sigma: sigma28/FLGM
… but only after associating with RNAP to form the holoenzyme. In the holoenzyme, the major sigma domains (sigma2, sigma3, and sigma4) are spread across the surface, with the promoter binding surfaces solvent exposed and appropriately positioned for simultaneous recognition of the -10 and -35 …
-
Autoregulatory and structural control of CaMKII substrate specificity
… protein kinase II (CaMKII) is a multimeric holoenzyme composed of 8–14 subunits from four closely related isoforms (α, β, γ, δ). CaMKII plays a strategic, multifunctional role in coupling the universal second messenger calcium with diverse cellular processes including metabolism, cell cycle …
-
Biochemical Studies of Ribonuclease P of Tetrahymena Thermophila
… been performed with the RNase P RNA subunit and holoenzymes from various sources. Experimental studies clearly reveal that the prokaryotic RNA subunit is involved in substrate binding, but that the eukaryotic RNA subunit alone is unable to bind substrate. Crosslinking experiments with the …
-
Purification of Aspartate Transcarbamoylase from Moraxella (Branhamella) catarrhalis
… catarrhalis, has been purified. The holoenzyme has a molecular mass of approximately 510kDa, harbors predominantly positive charges and is hydrophobic in nature. The holoenzyme possesses two subunits, a smaller one of 40 kDa and a larger one of 45 kDa. A third polypeptide has been …
-
Characterization of Moraxella bovis Aspartate Transcarbamoylase
… A, B, and C, based on their molecular weight, holoenzyme architecture, and enzyme kinetics. Moraxella bovis is a fastidious organism, the etiologic agent of infectious bovine keratoconjunctivitis (IBK). The M. bovis ATCase was purified and characterized for the first time. It is a class A …
-
Genetic and Biochemical Characterization of the Heteromeric Dihydroorotate Dehydrogenase From Bacillus Subtilis
… that the two proteins formed a heteromeric DHOD holoenzyme, an iron-sulfur flavoprotein which was determined by gel filtration to be a tetramer containing 2 mol PyrDI and 2 mol PyrDII. The two subunits were also overexpressed individually and purified. Overexpressed PyrDII formed inclusion bodies …
-
Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… of myosin light chain phosphatase (MLCP), a holoenzyme composed of a catalytic subunit (PP1cβ) and a regulatory subunit that targets the phosphatase to sarcomeric substrates. PPP1R12C is an atrial-enriched MLCP regulatory subunit that directs PP1cβ to the myosin regulatory light chain …
-
Structural and Functional Studies of Biotin-Dependent Carboxylases
… the first crystal structure of its 750-kDa α6β6 holoenzyme. This structure reveals the architecture of PCC's twelve catalytic domains and allows the mapping of its disease-associated gene mutations to predict their effects on enzyme stability and catalysis. We also identify and describe a new …
-
Purification and Characterization of Proteolytic Aspartate Transcarbamoylase (ATCase) from Burkholderia cepacia 25416 and Construction of a pyrB1 Knock-out Mutant
… activity gels showed that B. cepacia contained a holoenzyme pyrBC complex of 550 kDa comprised of 47 kDa pyrB and 45 kDa pyrC subunits. In the course of purifying the enzyme, trimeric subunits of 140 kDa and 120 kDa were observed as well as a unique proteolysis of the enzyme. The 47 kDa ATCase …
-
Studio della subunità epsilon della DNA polimerasi III di Escherichia coli: stabilità e interazione con la subunità polimerasica
… known DNA polymerases (Pol). DNA polymerase III holoenzyme is the major replicative polymerase of the Escherichia coli chromosome (Kornberg, 1982). This enzyme contains two Pol III cores that are held together by a t dimer (Studwell-Vaughan and O’Donnell, 1991). The core is composed of three …
-
Repression of telomerase components in isogenic normal, immortal and tumorigenic cells
… essential components of the active telomerase holoenzyme include the reverse transcriptase, hTERT, an RNA component (hTR) that has a template region for reverse transcription and the RNA binding and modifying protein, dyskerin. There is evidence indicating that these components may contribute …
-
Characterization of the Omega Subunit of Borrelia Burgdorferi’s RNA Polymerase
… carried out by RNA polymerase (RNAP). The RNAP holoenzyme consists of two ɑ subunits, a β subunit, and a β’ subunit. Additional proteins have been implicated in the function of RNAP including RpoZ (i.e. omega subunit, ω). Investigations of RpoZ homologues from other bacteria have suggested the …
-
The Role of Phosphorylation in Modulating PC4 Function as a General Cofactor
… TFIIH and a preassembled RNA polymerase II holoenzyme complex but not by the other GTFs or RNA polymerase II. Our finding suggests that phosphorylation of PC4 by TFIID, TFIIH or RNA polymerase II holoenzyme may cause a conformational change in the structure of PC4 that allows for …
-
Endogenous cyclic AMP-dependent phosphorylation in vitro of cytosolic proteins from Dictyostelium discoideum
… subunit prepared from the Dictyostelium holoenzyme form.
Page 1 of 4