Effects of exogenous and endogenous factors on appetite regulation in broiler chicks and Japanese quail

Understanding how appetite is regulated, via exogenous or endogenous factors, is essential to animal agriculture in order to maximize production capabilities, as well as in human medicine to generate ways to treat conditions such as eating disorders or obesity. The aim of this thesis was to evaluate the effects of ferulic acid (FA), an exogenous factor found within plant cells, and oxyntomodulin (OXM), an endogenous hormone generated in the gastrointestinal tract, on food intake in avian models, as well as elucidate the hypothalamic mechanisms responsible. In broiler chicks (Gallus gallus), FA administered peripherally (IP) resulted in a transient yet potent reduction of food intake. A behavior analysis revealed that FA-treated chicks defecated fewer times than control birds. Within the arcuate nucleus (ARC) there was an increase in c-Fos immunoreactivity, indicating neuronal activation, in FA-treated chicks. Within the hypothalamus, there was a decrease in mRNA abundance of galanin, ghrelin, melanocortin receptor 3, and pro-opiomelanocortin (POMC), however within the ARC there was a decrease in POMC and an increase in c-Fos mRNA after FA treatment. OXM, a proglucagon-derived peptide produced in the gastrointestinal tract, administered intracerebroventricularly (ICV) or IP in Japanese quail (Coturnix japonica), resulted in a decrease in food intake for 3 hours post-injection. There was an increase in c-Fos immunoreactivity within the ARC as well as the dorsomedial nucleus (DMN) in quail ICV injected with OXM. In conclusion, these novel data provide insights on the similarities and differences between factors that can affect appetite regulation via anorexigenic effects.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Animal and Poultry Sciences

Department dc:contributor.department

Animal and Poultry Sciences

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2021

Author dc:creator

• Halter, Bailey Anne

Chair dc:contributor.committeechair

• Gilbert, Elizabeth R.

Committee members dc:contributor.committeemember

• Campbell, Susan
• Cline, Mark A.

• Chicks
• Japanese quail
• ferulic acid
• oxyntomodulin
• arcuate nucleus

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

vt_gsexam:31531

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/103601