The significance of aberrant phenotypes and multidrug resistance for evaluation of the dynamics of minimal residual disease in acute leukemia patients /

With application of modern treatment most acute leukemia patients achieve complete clinical and morphological remission, however due to the persistence of submicroscopic level of leukemic cells some patients eventually relapse. The purpose of this study was to verify the hypothesis that the detection of aberrant phenotypes by flow cytometry may show leukemic cells to be resistant to treatment and therefore residing in the patient's bone marrow, even when intensive chemotherapy is applied. Multidrug resistance has been studied determining the expression of multidrug resistance related proteins (P-gp, MRP1) on the surface of leukemic cells and examining their function by detecting an accumulation of calcein within the cell. The response to treatment was assessed applying the original flow cytometric assay for minimal residual disease detection. We found that several aberrant phenotypes of patients with acute myelogenous leukemia (CD13+CD33+CD56+, CD5+, CD7+) are related to an increased multidrug resistance and through this mechanism influence a higher level of minimal residual disease. Aberrant phenotypes of patients with acute lymphoblastic leukemia did not correlate with parameters of multidrug resistance, yet several phenotypes were associated with a higher or lower minimal residual disease. These phenotypes influence the number of residing during treatment leukemic cells by biological mechanisms other than multidrug resistance. In summary, the assessment of aberrant leukemia associated phenotypes and multidrug resistance by flow cytometry can provide important prognostic information and help to identify groups of patients who would benefit from individualized treatment.

Grantor dc:publisher

Institutional Repository of Vilnius University

Year dc:date

2012

Author dc:creator

• Janeliūnienė, Miglė,

• acute leukemia ; aberrant phenotypes ; multidrug resistance ; minimal residual disease ; flow cytometry

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

eng

Repository record dc:identifier

https://repository.vu.lt/VU:ELABAETD2013828&prefLang=en_US

OAI identifier oai:identifier

oai:vu.lt:elaba:2013828