Analysis of Mouse EKLF/KLF2 E9.5 Double Knockout: Yolk Sac Morphology and Embryonic Erythroid Maturation

Krüppel-like factors (KLFs) are a family of transcription factors with 3 Cys2/His2 zinc fingers that regulate cell differentiation and developmental processes. EKLF is involved in primitive and definitive erythropoiesis; KLF2 is implicated in the development of primitive erythroid and endothelial cells of the vasculature. Using light and electron microscopy, the yolk sacs and dorsal aortae from EKLF/KLF2 double knockout (KO) E9.5 (embryonic day 9.5) were examined to determine whether these KLFs have compensatory functions in morphology of blood cells and vessels. EKLF/KLF2 double KO E9.5 erythroid, endothelial, and mesothelial cells had more severely abnormal morphology than WT and KLF2-/-. Flow cytometry and cytospins were used to determine maturational effects of single and EKLF/KLF2 double KO primitive erythroid cells double-labeled with anti-TER119 and anti-CD71. EKLF KO and EKLF/KLF2 double KO erythroid cells display defective erythroid maturation. EKLF and KLF2 have overlapping roles in the development of embryonic erythroid and endothelial cells.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Anatomy & Neurobiology

Year dc:date.available

2007

Author dc:creator

• Lung, Tina Kathy

Contributors dc:contributor

• Dr. Joyce A. Lloyd
• Dr. Jack L. Haar

• gene regulation
• embryo development
• hematopoiesis
• endothelial cell
• erythroid cell
• cell morphology
• Anatomy
• Medicine and Health Sciences
• Nervous System

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/1161

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-2160