STUDIES ON THE REACTION OF HIGH-DOSE HYDROXOCOBALAMIN AND ASCORBIC ACID WITH CARBON MONOXIDE: IMPLICATIONS FOR TREATMENT OF CARBON MONOXIDE POISONING

Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic acid caused a 5-fold increase in the CO2 concentration of the gas-out flow, in comparison to baseline and negative controls. These findings offer initial support for the potential use of a mixture of hydroxocobalamin and ascorbic acid as an injectable antidote for carbon monoxide poisoning.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Physiology

Year dc:date.available

2013

Author dc:creator

• Roderique, Joseph

Contributors dc:contributor

• Bruce Spiess
• Robert Diegelmann
• Roland Pittman

• Hydroxocobalamin
• Vitamin B12
• Carbon Monoxide
• Resonance Raman Spectroscopy
• Ascorbic Acid
• Antidote
• hemoglobin
• Life Sciences
• Physiology

Statement dc:rights

• © The Author

Identifier

https://scholarscompass.vcu.edu/etd/469

OAI identifier oai:identifier

oai:scholarscompass.vcu.edu:etd-1468