{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-1468"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-1468","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"STUDIES ON THE REACTION OF HIGH-DOSE HYDROXOCOBALAMIN AND ASCORBIC ACID WITH CARBON MONOXIDE: IMPLICATIONS FOR TREATMENT OF CARBON MONOXIDE POISONING","abstract":"Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic acid caused a 5-fold increase in the CO2 concentration of the gas-out flow, in comparison to baseline and negative controls. These findings offer initial support for the potential use of a mixture of hydroxocobalamin and ascorbic acid as an injectable antidote for carbon monoxide poisoning.","abstract_html":"Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic acid caused a 5-fold increase in the CO2 concentration of the gas-out flow, in comparison to baseline and negative controls. These findings offer initial support for the potential use of a mixture of hydroxocobalamin and ascorbic acid as an injectable antidote for carbon monoxide poisoning.","abstract_has_math":false,"creators":["Roderique, Joseph"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":["Bruce Spiess","Robert Diegelmann","Roland Pittman"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-04-10T07:00:00Z","date_published":"2013-04-10T07:00:00Z","updated_at":"2026-07-24T05:53:56Z","subjects":["Hydroxocobalamin","Vitamin B12","Carbon Monoxide","Resonance Raman Spectroscopy","Ascorbic Acid","Antidote","hemoglobin","Life Sciences","Physiology"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/469"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/469","href":"https://scholarscompass.vcu.edu/etd/469","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/SZJ2-CN93","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bruce Spiess","Robert Diegelmann","Roland Pittman"]},{"key":"dc:creator","label":"Author","values":["Roderique, Joseph"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2018-05-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Hydroxocobalamin","Vitamin B12","Carbon Monoxide","Resonance Raman Spectroscopy","Ascorbic Acid","Antidote","hemoglobin","Life Sciences","Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© The Author"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.25772/SZJ2-CN93","https://scholarscompass.vcu.edu/etd/469"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic acid caused a 5-fold increase in the CO2 concentration of the gas-out flow, in comparison to baseline and negative controls. These findings offer initial support for the potential use of a mixture of hydroxocobalamin and ascorbic acid as an injectable antidote for carbon monoxide poisoning."]},{"key":"dc:title","label":"Title","values":["STUDIES ON THE REACTION OF HIGH-DOSE HYDROXOCOBALAMIN AND ASCORBIC ACID WITH CARBON MONOXIDE: IMPLICATIONS FOR TREATMENT OF CARBON MONOXIDE POISONING"]}]}],"canonical_facts":{"dc:contributor":["Bruce Spiess","Robert Diegelmann","Roland Pittman"],"dc:creator":["Roderique, Joseph"],"dc:date.available":["2018-05-09T07:00:00Z"],"dc:description.abstract":["Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic acid caused a 5-fold increase in the CO2 concentration of the gas-out flow, in comparison to baseline and negative controls. These findings offer initial support for the potential use of a mixture of hydroxocobalamin and ascorbic acid as an injectable antidote for carbon monoxide poisoning."],"dc:identifier":["https://doi.org/10.25772/SZJ2-CN93","https://scholarscompass.vcu.edu/etd/469"],"dc:rights":["© The Author"],"dc:subject":["Hydroxocobalamin","Vitamin B12","Carbon Monoxide","Resonance Raman Spectroscopy","Ascorbic Acid","Antidote","hemoglobin","Life Sciences","Physiology"],"dc:title":["STUDIES ON THE REACTION OF HIGH-DOSE HYDROXOCOBALAMIN AND ASCORBIC ACID WITH CARBON MONOXIDE: IMPLICATIONS FOR TREATMENT OF CARBON MONOXIDE POISONING"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:53:56Z"}