Abstract
dc:description.abstractImmunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- Thesis
- Discipline thesis:degree_discipline
- Physics and Applied Physics
- Year dc:date.available
- 2011
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- MUTISYA, STEPHEN
- Contributors dc:contributor
-
- MASSIMO BERTINO
Subjects
dc:subject × 2Rights
dc:rights- Statement dc:rights
-
- © The Author
Identifiers
dc:identifier.*- Identifier
- https://scholarscompass.vcu.edu/etd/231
- OAI identifier oai:identifier
- oai:scholarscompass.vcu.edu:etd-1230