{"id":{"repo_id":"vcu","oai_identifier":"oai:scholarscompass.vcu.edu:etd-1230"},"canonical_url":"https://search.dev.ndltd.org/etd/vcu/oai:scholarscompass.vcu.edu:etd-1230","repository":{"repo_id":"vcu","name":"Virginia Commonwealth University","base_url":"https://scholarscompass.vcu.edu/do/oai/"},"display":{"title":"SYNTHESIS OF NANOCOMPOSITES BY LASER ABLATION","abstract":"Immunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery.","abstract_html":"Immunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery.","abstract_has_math":false,"creators":["MUTISYA, STEPHEN"],"institution":null,"degree_name":"Master of Science","degree_level":"Thesis","degree_discipline":"Physics and Applied Physics","degree_department":null,"school":null,"contributors":["MASSIMO BERTINO"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-12T07:00:00Z","date_published":"2011-05-12T07:00:00Z","updated_at":"2026-07-24T05:53:41Z","subjects":["Physical Sciences and Mathematics","Physics"],"languages":[],"rights":["© The Author"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarscompass.vcu.edu/etd/231"],"render_values":[{"text":"https://scholarscompass.vcu.edu/etd/231","href":"https://scholarscompass.vcu.edu/etd/231","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25772/QTMH-8T61","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["MASSIMO BERTINO"]},{"key":"dc:creator","label":"Author","values":["MUTISYA, STEPHEN"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-05-13T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics and Applied Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Physical Sciences and Mathematics","Physics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© The Author"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.25772/QTMH-8T61","https://scholarscompass.vcu.edu/etd/231"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Immunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery."]},{"key":"dc:title","label":"Title","values":["SYNTHESIS OF NANOCOMPOSITES BY LASER ABLATION"]}]}],"canonical_facts":{"dc:contributor":["MASSIMO BERTINO"],"dc:creator":["MUTISYA, STEPHEN"],"dc:date.available":["2021-05-13T07:00:00Z"],"dc:description.abstract":["Immunoglobulin E (IgE)-conjugated gold nanoparticles were produced via in situ conjugation of gold nanoparticles with immunoglobulin E by laser ablation of Au in a liquid solution. The colloidal stability and the size distribution of the resulting bio-nanoconjugates were examined with UV-Visible spectroscopy (UV-Vis), dynamic light scattering (DLS), and transmission electron microscopy (TEM). These techniques showed that the Au nanoparticles in aqueous solutions were highly monodispersed spherical particles with a very narrow size distribution. The particles net diameter using TEM, was found to be D5 =3.8±0.9nm and D10 =4.7±1.3nm while the hydrodynamic diameter obtained with DLS was found to be h5 D =171±12nm , h10 D =164±18nm for 5min and 10min laser ablation time respectively. Enzyme-Linked immunosorbent Assay (ELISA) and flow cytometry measurements of the conjugates confirmed that the gold-bound protein remained biologically active, thus paving the way for the application of these nanoparticles in immuno-diagnostics, particularly in tumor-targeted drug delivery."],"dc:identifier":["https://doi.org/10.25772/QTMH-8T61","https://scholarscompass.vcu.edu/etd/231"],"dc:rights":["© The Author"],"dc:subject":["Physical Sciences and Mathematics","Physics"],"dc:title":["SYNTHESIS OF NANOCOMPOSITES BY LASER ABLATION"],"thesis:degree_discipline":["Physics and Applied Physics"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T05:53:41Z"}