Role of Selected Plasma MicroRNAs as Diagnostic and Prognostic Biomarkers in Myelodysplastic Syndrome // Роля на подбрани плазмени микроРНК-и като диагностични и прогностични маркери при миелодиспластичен синдром

This thesis presents an original study on the role of five circulating microRNAs (miR-22, miR-144, miR-16, miR-451a, and let-7a) as potential diagnostic and prognostic biomarkers in myelodysplastic syndrome (MDS). The study aims to investigate the expression of these five microRNAs in the plasma of patients with MDS and healthy controls, and to analyse their correlation with clinical, laboratory, and prognostic parameters, including R-IPSS risk groups and bone marrow blast percentage. The study is prospective and includes 40 patients with confirmed MDS and 10 healthy controls. MicroRNA expression was analysed using Real-Time PCR, and statistical analysis was performed. The results show significantly lower levels of miR-16, miR-144, miR-451a, and let-7a in MDS patients, with miR-451a demonstrating the highest diagnostic value. MiR-144 and miR-451a correlate with cytogenetic risk, while let-7a is associated with blast percentage and R-IPSS. LASSO analysis identified miR-22 and miR-451a as the strongest predictors for distinguishing patients from healthy controls. Correlation analysis revealed associations between the microRNAs and biochemical markers such as LDH and ferritin. This study contributes to a better understanding of the role of microRNAs in MDS pathogenesis and highlights their potential future application in clinical practice as non-invasive biomarkers.

Grantor dc:publisher

Medical University of Varna

Year dc:date

2025

Authors dc:creator

• Atanasova, Svilena
• Атанасова, Свилена

• biomarkers
• myelodysplastic syndrome (MDS)
• plasma
• Хематология / Haematology

Repository record dc:identifier

http://repository.mu-varna.bg/handle/nls/4113

OAI identifier oai:identifier

oai:repository.mu-varna.bg:nls/4113