A comparison of community composition analyses for the assessment of responses to wood-ash soil amendment by free-living nematodes

Land-use changes can have far-reaching consequences for resident communities and ecosystem functioning. Developing appropriate assessment methods to observe and quantify this change is an important application of community ecology. Here I compare four methods of community assessment for free-living soil nematodes under forest harvesting disturbance and wood ash application. Neither morphological assessment (richness, abundance, diversity) nor molecular assessment (morpho-richness using T-RFLP) was responsive to experimental treatments. Trait-based approaches (Maturity Index (MI) and Body Size Spectra (BSS)) were more sensitive to forest harvest and wood-ash amendment treatments. The efficacy of these methods was also qualitatively compared. Of all methods, the BSS were found to be the most informative and easiest to implement. Morphological assessment and the MI rely strongly on rare taxonomic expertise and T-RFLP requires considerable optimisation to be effective. The use of trait-based approaches for soil fauna is advocated as an accessible tool for community ecologists, especially those interested in taxonomically difficult groups.

Name thesis:degree_name

M Sc

Discipline thesis:degree_discipline

Biology

Grantor dc:publisher

The University of Western Ontario

Year dc:date.issued

2014

Author dc:creator

• George, Paul B.L.

Advisor dc:contributor.advisor

• Zoe Lindo

• Soil fauna
• nematodes
• community ecology
• taxnonomy
• body size
• Maturity Index
• T-RFLP
• forestry

Language dc:language.iso

en_ca

Handle dc:identifier.uri

https://hdl.handle.net/20.500.14721/35502

OAI identifier oai:identifier

oai:uwo.scholaris.ca:20.500.14721/35502