Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"T-RFLP"”.
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic Assignment Tool (PAT). Species belonging to the Class Bacilli were the most abundant …
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A comparison of community composition analyses for the assessment of responses to wood-ash soil amendment by free-living nematodes
… molecular assessment (morpho-richness using T-RFLP) was responsive to experimental treatments. Trait-based approaches (Maturity Index (MI) and Body Size Spectra (BSS)) were more sensitive to forest harvest and wood-ash amendment treatments. The efficacy of these methods was also qualitatively …
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Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors
… restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of delivery on ileal Lactobacillus diversity. …
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To study the fitness of Bacillus thuringiensis to survive in natural and artificial, terrestrial ecosystems
… thuringiensis. The three techniques of DGGE, T-RFLP and MLST were compared for the first time to judge whether the fingerprinting and typing techniques provide the same level of resolution in the structure of the bacterial communities. The techniques resulted in interesting findings: (a) the …
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Intact soil core experiments reveal that temperature and depth influence microbial community function and impact the fate of nitrogenous fertilizer amendments
… structure and function were assessed by T-RFLP and qPCR, respectively. Comparison of 16S rRNA based T-RFLP total community profiles revealed a distinct temporal succession across soil depths, with shifts in community structure corresponding to changes in soil NO3- and NH4+ concentration. …
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Identification of endophytic bacterial communities associated with South African crops: sorghum bicolor (L. Moench), pennisetum glaucum and arachis villosulicarpa
… retriction fragment length polymorphism (t- RFLP) and next generation sequencing (NGS). The first objective of this study was to investigate the effect of different DNA extraction protocols on mDNA yield and quality, as well as the diversity of endophytic bacteria retrieved from root and stem …
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Seasonal dynamics of edaphic bacterial communities in the hyper-arid Namib desert
… combination with community fingerprinting by T-RFLP analyses and micro environmental characterization. The short-term study was conducted on three distinct locations in the Namib Desert gravel plains. Soil bacterial communities were found to be more similar within habitats than between habitats, …
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Struktur und Funktion von Fermentierern und Methanogenen in einem sauren Niedermoor
… Restriktionsfragmentlängenpolymorphismus [T-RFLP]- Analyse und Genbibliotheken) abgeschätzt. Säure-tolerante Enterobacteriaceae-, Burkholderiaceae- und Pseudomonadaceae-verwandte Arten wurden aus dem Niedermoor isoliert. Arten von Neisseriaceae, Enterobacteriaceae, Bacteroidetes, …
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Seasonal dynamics of edaphic bacterial communities in the hyper-arid namib desert
… combination with community fingerprinting by T-RFLP analyses and microenvironmental characterization. The short-term study was conducted on three distinct locations in the Namib Desert gravel plains. Soil bacterial communities were found to be more similar within habitats than between habitats, …
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Mikrobielle Ökologie N-Acyl-L-Homoserinlacton-produzierender Bakterien in der Rhizosphäre von Tomatenpflanzen
… restrictions fragment length polymorphism (t-RFLP)-techniques were used to examinate shifts of the bacterial population in the rhizosphere on tomato plants. The effective in situ production and spreading of AHL on tomato roots was demonstrated with P. putida IsoF using an AHL-sensor strain P. …
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Introducing the starlet sea anemone Nematostella vectensis as a model for investigating microbial mediation of health and disease in hexacorals
… fragment length polymorphism analysis (T- RFLP), fluorescent in situ hybridization (FISH) and cultivation-based analysis. N. vectensis microbiomes revealed the presence of a novel lineage of epsilon-Proteobacteria associated with animals collected from salt marshes in Sippewissett …
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Microbial community analysis for denitrifying biofilters
… fingerprint techniques using ARISA and nosZ t-RFLP were also developed to provide high throughput bacterial and denitrifying community data. The spatial structure of microbial communities in a biofilter was characterized using mapping of ecological metrics, MDS plots, and a new geostatistical …
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Molekularbiologische Analyse mikrobieller Gemeinschaften in Talsperrensedimenten
… Mittels Fingerprinttechniken (DGGE, T-RFLP, ARISA) konnten komplexe Muster der mikrobiellen Diversität erzeugt werden. Dabei konnten die Ergebnisse der 16S rDNA Analyse bestätigt werden. Durch die verwendeten Methoden konnte eine komplexe mikrobielle Diversität in den Sedimenten …
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Landscape history and contemporary environmental drivers of microbial community structure and function
… snow packs and hillslope ground ice. Using T-RFLP 16S rRNA gene profiles of microbial communities, I analyzed the contribution of till and environmental factors to community similarity, and assessed the functional potential of the microbial community using extracellular enzyme activity assays. …
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Determining fecal bacterial profiles of a human-habituated wild chimpanzee population in Mahale Mountains National Park, Tanzania
… Fragment Length Polymorphism (T-RFLP) of amplified 16S rRNA genes to determine bacterial diversity present. Between 1 and 14 terminal-restriction fragments (T-RFs) were observed in each sample. A total of 26 unique T-RFs were produced from the samples and ranged in size from 92 to …
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Fungal Biofilm Colonization and Succession on Artificial Reefs in the North-Central Gulf of Mexico
… DNA. Terminal fragment length polymorphism (T-RFLP) analysis of the ITS region was used to infer variation in fungal community structure. Fungal species were further characterized by performing morphological species identification as well as ITS gene sequencing. Significant temporal and …
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DEPTH AND TIME RELATED VARIATIONS OF MICROBIAL COMMUNIITES IN AN EMERGENT FRESHWATER WETLAND
… Restriction Fragment Length Polymorphism (T-RFLP) of 16S-rRNA. Analysis of variance (ANOVA) found significant differences were found between the environmental parameters in regards to site, depth, and season. Three physical-chemical variables (WC, OM, and redox) were different between sites, …
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Stream microbial communities along an agricultural gradient
… restriction fragment length polymorphism (T-RFLP) on the small ribosomal subunit 16S rRNA assessed microbial diversity; and quantitative PCR (qPCR) on amoA, a subunit of the nitrifying gene ammonia monooxygenase, measured nitrifier abundance. A geographic information system (ArcGIS) was used …
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Surfactina desidratada como agente potencializador da biorremediação de solos contaminados com petróleo
… microbianas foi estudada pela análise de T-RFLP multiplex dos grupos de Bacteria, Fungi e Archaea. Os resultados demonstraram a ocorrência de impacto negativo do petróleo nas comunidades microbianas de todos os microcosmos em ambos os tipos de solo, no início do período de incubação, e …
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Development and application of a microbial reliability model to analyze denitrifying biofilter stability
… restriction fragment length polymorphism (T-RFLP). This allowed us to get a better understanding of how to model the microbial populations as functional components in the system. In order to quickly and easily apply these methods to microbial fingerprinting data, we have developed the …
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