Elucidation of the Mechanism of Action of a Cell Line Selective Toxin

A hallmark of targeted cancer therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic to the same four of 12 lung cancer cell lines at low nanomolar concentrations. Sensitive cell lines expressed cytochrome P450 (CYP) 4F11, which metabolized the compounds into irreversible stearoyl CoA desaturase (SCD) inhibitors. SCD has been recognized as a promising biological target in cancer and metabolic disease. However, SCD is essential to sebocytes, and accordingly SCD inhibitors cause skin toxicity. Mouse sebocytes were unable to activate the benzothiazoles or oxalamides into SCD inhibitors, providing a therapeutic window for inhibiting SCD in vivo. We thus offer a strategy to target SCD in cancer by taking advantage of high CYP expression in a subset of tumors.

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Biological Chemistry

Grantor

UT Southwestern Medical Center

Year dc:date.issued

2019

Author dc:creator

• Theodoropoulos, Panayotis Christos

Advisor dc:contributor.advisor

• Yu, Hongtao

Committee members dc:contributor.committeemember

• Nijhawan, Deepak
• Brown, Michael S.
• Ready, Joseph M.
• Chen, Zhijian J.

• Antineoplastic Agents
• Benzothiazoles
• Drug Discovery
• Lung Neoplasms
• Oxamic Acid
• Stearoyl-CoA Desaturase

Language dc:language.iso

en

Identifier

1103324615

OAI record oai:identifier

https://utswmed-ir.tdl.org/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Autswmed-ir.tdl.org%3A2152.5%2F6623