Deconvolute Brain Tumor Genomic Alterations Based On Dna Methylation

<p>Molecular classification based on mutations, expression subtypes, and copy number variants has improved diagnosis and treatment decision-making for patients with brain tumors, particularly malignant gliomas. However, the association between epigenetic signature and genetic alterations is poorly understood. For example, mutation of isocitrate dehydrogenase (<em>IDH</em>) is associated with genome-wide hypermethylation of CpG islands in gliomas. But other subtype-associated alterations, including telomerase reverse transcriptase (<em>TERT</em>)<em> </em>promoter mutation, alpha thalassemia/mental retardation syndrome X-linked (<em>ATRX</em>) mutation, chromosome 1p19q co-deletion (chr1p19q codel), and gene expression subtypes, have yet to be associated with any epigenetic signature. Therefore, we hypothesized that DNA methylation signatures can classify gliomas based on these alterations and give insight into subgroup characteristics. Machine learning models, including elastic net and random forest, were used to predict somatic mutations of <em>IDH</em>, <em>TERT</em>p, and <em>ATRX</em>, chr1p19q codel, and gene expression subtype of gliomas. Data from the NOA-04 randomized phase III trial were used for external validation. In total,<strong> </strong>926 cases from The Cancer Genome Atlas were included in this study. Prediction accuracies for <em>IDH</em>, <em>TERT</em>p, and <em>ATRX</em> mutations, and chr1p19q codel were 100%, 98.3%, 90.48%, and 99.21%, respectively in test set. Accuracy for gene expression subtype prediction was 72.2%. The methylation-based prediction models for both <em>ATRX</em> and chr1p19q codel statuses proved superior to conventional assays for these biomarkers. Similarly, characteristic alterations associated with gene expression subtypes were better discriminated using methylation compared to transcriptome-based classification. DNA methylation signatures accurately predicted somatic alterations and improved over existing classifiers. The established Unified Diagnostic Pipeline (UniD) is a rapid and cost-effective diagnostic platform of genomic alterations and gene expression subtypes at initial clinical diagnosis and improves over individual assays currently in clinical use. The significant relationship between genetic alterations and epigenetic signatures indicates the broad applicability of our approach to other malignancies.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation (PhD)

Year dc:date.available

2019

Authors dc:creator

• Yang, Jie
• <p>0000-0003-2241-5066</p>

Contributors dc:contributor

• Erik Sulman
• Jason Huse
• Krishna Bhat

• gliomas
• DNA methylation
• genomic alterations
• pipeline
• Medicine and Health Sciences

Repository record dc:identifier

https://digitalcommons.library.tmc.edu/utgsbs_dissertations/984

OAI identifier oai:identifier

oai:digitalcommons.library.tmc.edu:utgsbs_dissertations-2029